Diffraction-unlimited fluorescence imaging allows the visualization of intact, strongly
heterogeneous systems at unprecedented levels of detail. Beyond the acquisition of detailed …

In commonly studied GFP chromophore analogues such as 4-(4-hydroxybenzylidene)-1, 2-
dimethyl-1H-imidazol-5 (4H)-one (PHBDI), the dominant photoinduced processes are cis …

Most GFP-like fluorescent proteins exhibit small Stokes shifts (10–45 nm) due to rigidity of
the chromophore environment that excludes non-fluorescent relaxation to a ground state. An …

Optoacoustic (photoacoustic) imaging has seen marked advances in detection and data
analysis, but there is less progress in understanding the photophysics of common …

Single-molecule superresolution microscopy is a powerful tool for the study of biological
structures on size scales smaller than the optical diffraction limit. Imaging samples at …

Fluorescent proteins (FPs) are powerful tools that permit real-time visualization of cellular
processes. The utility of a given FP for a specific experiment depends strongly on its effective …

Anthozoa-class red fluorescent proteins (RFPs) are frequently used as biological markers,
with far-red (λem∼ 600–700 nm) emitting variants sought for whole-animal imaging …

Optoacoustic (photoacoustic) imaging has seen marked technological advances in detection
and data analysis, but there is less progress in understanding the photophysics of …

Chromoprotein asFP595 and its A143G variant called kindling fluorescent protein (KFP) are
among the chronologically first species for which trans–cis chromophore isomerization has …

Red fluorescent proteins (RFPs) are widely used probes for monitoring subcellular
processes with extremely high spatial and temporal precision. In this work, we employed …