[HTML][HTML] Application of human mesenchymal and pluripotent stem cell microcarrier cultures in cellular therapy: achievements and future direction

AKL Chen, S Reuveny, SKW Oh - Biotechnology advances, 2013 - Elsevier
Mesenchymal stem cells (MSCs) have recently made significant progress with multiple
clinical trials targeting modulation of immune responses, regeneration of bone, cartilage …

[HTML][HTML] Progress and challenges in large-scale expansion of human pluripotent stem cells

C Kropp, D Massai, R Zweigerdt - Process Biochemistry, 2017 - Elsevier
The constant supply of high cell numbers generated by defined, robust, and economically
viable culture processes is indispensable for the envisioned application of human …

A scalable system for production of functional pancreatic progenitors from human embryonic stem cells

TC Schulz, HY Young, AD Agulnick, MJ Babin… - PloS one, 2012 - journals.plos.org
Development of a human embryonic stem cell (hESC)-based therapy for type 1 diabetes will
require the translation of proof-of-principle concepts into a scalable, controlled, and …

A fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation

Y Lei, DV Schaffer - … of the National Academy of Sciences, 2013 - National Acad Sciences
Human pluripotent stem cells (hPSCs), including human embryonic stem cells and induced
pluripotent stem cells, are promising for numerous biomedical applications, such as cell …

Process engineering of human pluripotent stem cells for clinical application

M Serra, C Brito, C Correia, PM Alves - Trends in biotechnology, 2012 - cell.com
Human pluripotent stem cells (hPSCs), including embryonic and induced pluripotent stem
cells, constitute an extremely attractive tool for cell therapy. However, flexible platforms for …

Controlling expansion and cardiomyogenic differentiation of human pluripotent stem cells in scalable suspension culture

H Kempf, R Olmer, C Kropp, M Rückert, M Jara-Avaca… - Stem cell reports, 2014 - cell.com
To harness the potential of human pluripotent stem cells (hPSCs), an abundant supply of
their progenies is required. Here, hPSC expansion as matrix-independent aggregates in …

The use of nanofibrillar cellulose hydrogel as a flexible three-dimensional model to culture human pluripotent stem cells

YR Lou, L Kanninen, T Kuisma, J Niklander… - Stem cells and …, 2014 - liebertpub.com
Human embryonic stem cells and induced pluripotent stem cells have great potential in
research and therapies. The current in vitro culture systems for human pluripotent stem cells …

Concise review: the evolution of human pluripotent stem cell culture: from feeder cells to synthetic coatings

LG Villa-Diaz, AM Ross, J Lahann, PH Krebsbach - Stem cells, 2013 - academic.oup.com
Current practices to maintain human pluripotent stem cells (hPSCs), which include induced
pluripotent stem cells and embryonic stem cells, in an undifferentiated state typically depend …

Scalable expansion of human pluripotent stem cells in suspension culture

R Zweigerdt, R Olmer, H Singh, A Haverich, U Martin - Nature protocols, 2011 - nature.com
Routine commercial and clinical applications of human pluripotent stem cells (hPSCs) and
their progenies will require increasing cell quantities that cannot be provided by …

[HTML][HTML] Development of a scalable suspension culture for cardiac differentiation from human pluripotent stem cells

VC Chen, J Ye, P Shukla, G Hua, D Chen, Z Lin, J Liu… - Stem cell …, 2015 - Elsevier
To meet the need of a large quantity of hPSC-derived cardiomyocytes (CM) for pre-clinical
and clinical studies, a robust and scalable differentiation system for CM production is …