Long-read sequencing–a powerful tool in viral transcriptome research
Long-read sequencing (LRS) has become increasingly popular due to its strengths in de
novo assembly and in resolving complex DNA regions as well as in determining full-length …
novo assembly and in resolving complex DNA regions as well as in determining full-length …
Getting the entire message: progress in isoform sequencing
The advent of second-generation sequencing and its application to RNA sequencing have
revolutionized the field of genomics by allowing quantification of gene expression, as well as …
revolutionized the field of genomics by allowing quantification of gene expression, as well as …
Iso-Seq allows genome-independent transcriptome profiling of grape berry development
A Minio, M Massonnet… - G3: Genes …, 2019 - academic.oup.com
Transcriptomics has been widely applied to study grape berry development. With few
exceptions, transcriptomic studies in grape are performed using the available genome …
exceptions, transcriptomic studies in grape are performed using the available genome …
Long-read sequencing uncovers a complex transcriptome topology in varicella zoster virus
Background Varicella zoster virus (VZV) is a human pathogenic alphaherpesvirus harboring
a relatively large DNA molecule. The VZV transcriptome has already been analyzed by …
a relatively large DNA molecule. The VZV transcriptome has already been analyzed by …
Long-read sequencing of human cytomegalovirus transcriptome reveals RNA isoforms carrying distinct coding potentials
The human cytomegalovirus (HCMV) is a ubiquitous, human pathogenic herpesvirus. The
complete viral genome is transcriptionally active during infection; however, a large part of its …
complete viral genome is transcriptionally active during infection; however, a large part of its …
Long-read isoform sequencing reveals a hidden complexity of the transcriptional landscape of herpes simplex virus type 1
In this study, we used the amplified isoform sequencing technique from Pacific Biosciences
to characterize the poly (A)+ fraction of the lytic transcriptome of the herpes simplex virus …
to characterize the poly (A)+ fraction of the lytic transcriptome of the herpes simplex virus …
Identification of herpesvirus transcripts from genomic regions around the replication origins
Long-read sequencing (LRS) techniques enable the identification of full-length RNA
molecules in a single run eliminating the need for additional assembly steps. LRS research …
molecules in a single run eliminating the need for additional assembly steps. LRS research …
Transcriptional and epi-transcriptional dynamics of SARS-CoV-2 during cellular infection
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) uses subgenomic RNA
(sgRNA) to produce viral proteins for replication and immune evasion. We apply long-read …
(sgRNA) to produce viral proteins for replication and immune evasion. We apply long-read …
Multi-platform sequencing approach reveals a novel transcriptome profile in pseudorabies virus
Third-generation sequencing is an emerging technology that is capable of solving several
problems that earlier approaches were not able to, including the identification of transcripts …
problems that earlier approaches were not able to, including the identification of transcripts …
Third-generation sequencing reveals extensive polycistronism and transcriptional overlapping in a baculovirus
The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is an insect-pathogen
baculovirus. In this study, we applied the Oxford Nanopore Technologies platform for the …
baculovirus. In this study, we applied the Oxford Nanopore Technologies platform for the …