Cell biologists strive to characterize molecular interactions directly in the intracellular
environment. The intrinsic resolution of optical microscopy, however, allows visualization of …

For a little more than a century, fluorescence microscopy has been an essential source of
major discoveries in cell biology. Recent developments improved both visualization and …

The contactless heating capacity of magnetic nanoparticles (MNPs) has been exploited in
fields such as hyperthermia cancer therapy, catalysis, and enzymatic thermal regulation …

Dye photobleaching is a major constraint of fluorescence readout within a range of
applications. In this study, we investigated the influence of photobleaching in fluorescence …

Specific binding proteins are crucial for the correct spatiotemporal expression of mRNA. To
understand this process, a method is required to characterize RNA-protein interactions in …

We successfully demonstrate the effectiveness of two-photon fluorescence cross-correlation
spectroscopy (TPCCS) to study the complex binding stoichiometry of calmodulin (CaM) and …

The red fluorescent protein mCherry is of considerable interest for fluorescence fluctuation
spectroscopy (FFS), because the wide separation in color between mCherry and green …

We introduce a new analysis technique for fluorescence fluctuation data. Time-integrated
fluorescence cumulant analysis (TIFCA) extracts information from the cumulants of the …

Pulsed interleaved excitation (PIE) is the methodology of interleaved or alternating excitation
of different fluorophores on the nanosecond timescale, which allows quasi-simultaneous, yet …

Fluorescence cross-correlation spectroscopy (FCCS) is a method that measures the
temporal fluorescence fluctuations coming from two differently labeled molecules diffusing …