Paramagnetic chemical probes have been used in electron paramagnetic resonance (EPR)
and nuclear magnetic resonance (NMR) spectroscopy for more than four decades. Recent …

Paramagnetic centers in biomolecules, such as specific metal ions that are bound to a
protein, affect the nuclei in their surrounding in various ways. One of these effects is the …

Pseudocontact shifts in biomolecular NMR using paramagnetic metal tags Page 1 1
Pseudocontact shifts in biomolecular NMR using paramagnetic metal tags Christoph …

The two-component dengue virus NS2B-NS3 protease (DEN NS2B-NS3pro) is an
established drug target, but inhibitor design is hampered by the lack of a crystal structure of …

Highlights•Protein engineering with uAAs is a valuable technique with applications in the
study of protein function, protein modification and biophysics.•The unique chemistry of some …

Structural studies of proteins and protein–ligand complexes by nuclear magnetic resonance
(NMR) spectroscopy can be greatly enhanced by site-specific attachment of lanthanide ions …

In structural studies by NMR, pseudocontact shifts (PCSs) provide both angular and distance
information. For proteins, incorporation of a di-histidine (diHis) motif, coordinated to Co2+ …

The advent of efficient systems using genetic encoding for the site-specific incorporation of
unnatural amino acids (UAA) into proteins has opened countless new possibilities for …

Over the last years protein engineering using non-standard amino acids has gained
increasing attention. As a result, improved methods are now available, enabling the efficient …

Pseudocontact shifts (PCS) from paramagnetic lanthanide ions present powerful long-range
structural restraints for structural biology by NMR spectroscopy, but site-specific tagging of …