Nucleases cleave the phosphodiester bonds of nucleic acids and may be endo or exo,
DNase or RNase, topoisomerases, recombinases, ribozymes, or RNA splicing enzymes. In …

RNase E is an essential endonuclease that is abundant in many bacteria and plays an
important part in all aspects of RNA metabolism. It functions as part of a large …

The development of electrospray ionization coupled to mass spectrometry has enabled the
analysis of very large intact protein complexes, even when they are held together by weak …

Regulation, recognition and cell signaling involve the coordinated actions of many players.
Signaling scaffolds, with their ability to bring together proteins belonging to common and/or …

The coordinated regulation of gene expression is required for homeostasis, growth and
development in all organisms. Such coordination may be partly achieved at the level of …

The finding that a large fraction of proteins (over 30%) in eukaryotic cells lack a unique three-
dimensional structure but are functional has forced the scientific community to review its …

It is established that noncovalent complexes can be maintained both during and after
electrospray and that assemblies of increasing size and complexity often lead to broadened …

The hydrolytic endoribonuclease RNase E, which is widely distributed in bacteria and
plants, plays key roles in mRNA degradation and RNA processing in Escherichia coli. The …

Instability is a fundamental property of mRNA that is necessary for the regulation of gene
expression. In E. coli, the turnover of mRNA involves multiple, redundant pathways involving …

Summary Ribonuclease E (RN ase E) of E scherichia coli, which is the founding member of
a widespread family of proteins in bacteria and chloroplasts, is a fascinating enzyme that still …