The use of biomarkers as early warning systems in the evaluation of disease risk has
increased markedly in the last decade. Biomarkers are indicators of typical biological …

Several metabolomic software programs provide methods for peak picking, retention time
alignment and quantification of metabolite features in LC/MS-based metabolomics …

Circadian rhythms are essential to health. Their disruption is associated with metabolic
diseases in experimental animals and man [1–3]. Local metabolic rhythms represent an …

We assess the reliability of isobaric-tags for relative and absolute quantitation (iTRAQ),
based on different types of replicate analyses taking into account technical, experimental …

We describe a proteomics method for determining the subcellular localization of membrane
proteins. Organelles are partially separated using centrifugation through self-generating …

Among the targets of the repressive splicing regulator, polypyrimidine tract binding protein
(PTB) is its own pre-mRNA, where PTB-induced exon 11 skipping produces an RNA …

DIGE is a protein labelling and separation technique allowing quantitative proteomics of two
or more samples by optical fluorescence detection of differentially labelled proteins that are …

Mice lacking the expression of proteolipid protein (PLP)/DM20 in oligodendrocytes provide a
genuine model for spastic paraplegia (SPG-2). Their axons are well myelinated but exhibit …

Quantitative proteomics is the workhorse of the modern proteomics initiative. The gel-based
and MuDPIT approaches have facilitated vital advances in the measurement of protein …

Two dimensional gel electrophoresis has been one of the techniques most used for protein
separation in proteomics experiments and still continues to be so for some species such as …