[HTML][HTML] Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies

RC Rodrigues, Á Berenguer-Murcia… - Biotechnology …, 2021 - Elsevier
The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …

Improvement of enzyme activity, stability and selectivity via immobilization techniques

C Mateo, JM Palomo, G Fernandez-Lorente… - Enzyme and microbial …, 2007 - Elsevier
In spite of their excellent catalytic properties, enzyme properties have to be usually improved
before their implementation at industrial scale (where many cycles of high yield processes …

Enzyme stability and stabilization—aqueous and non-aqueous environment

PV Iyer, L Ananthanarayan - Process biochemistry, 2008 - Elsevier
Enzyme stabilization has notable importance due to increasing number of enzyme
applications. Stabilization of enzymes in order to realize their full potential as catalysts is …

Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation

R Fernandez-Lafuente - Enzyme and microbial technology, 2009 - Elsevier
The moderate stability of enzymes is one of the main drawbacks that hinder general
implementation of these interesting biocatalysts at industrial scale. An especially complex …

Coupling chemical modification and immobilization to improve the catalytic performance of enzymes

RC Rodrigues, Á Berenguer‐Murcia… - Advanced Synthesis …, 2011 - Wiley Online Library
Chemical modification and immobilization of enzymes have been usually considered
unrelated tools to improve biocatalyst features. However, there are many examples where a …

Enhancing the functional properties of thermophilic enzymes by chemical modification and immobilization

DA Cowan, R Fernandez-Lafuente - Enzyme and Microbial Technology, 2011 - Elsevier
The immobilization of proteins (mostly typically enzymes) onto solid supports is mature
technology and has been used successfully to enhance biocatalytic processes in a wide …

Catalase immobilization—A review

AG Grigoras - Biochemical Engineering Journal, 2017 - Elsevier
This interdisciplinary review covers the updated information about the natural and the
synthetic materials based on polymers or low molecular compounds, organic or inorganic …

Silica encapsulated catalase@ metal-organic framework composite: A highly stable and recyclable biocatalyst

J Cui, Y Feng, S Jia - Chemical Engineering Journal, 2018 - Elsevier
Metal-organic frameworks (MOFs) have recently emerged as a promising candidate for the
immobilization of enzymes due to their diversified structures and porosity. However, it is …

Enzymes@ ZIF-8 nanocomposites with protection nanocoating: stability and acid-resistant evaluation

Y Feng, L Zhong, M Bilal, Z Tan, Y Hou, S Jia, J Cui - Polymers, 2018 - mdpi.com
Zeolitic imidazole framework-8 (ZIF-8) with tunable pore sizes and high surface areas have
recently emerged as a promising support for immobilizing enzymes. However, the instability …

Catalase immobilization: Current knowledge, key insights, applications, and future prospects-A review

S Abdalbagemohammedabdalsadeg, B Xiao… - International Journal of …, 2024 - Elsevier
Catalase (CAT), a ubiquitous enzyme in all oxygen-exposed organisms, effectively
decomposes hydrogen peroxide (H 2 O 2), a harmful by-product, into water and oxygen …