Paramagnetic chemical probes have been used in electron paramagnetic resonance (EPR)
and nuclear magnetic resonance (NMR) spectroscopy for more than four decades. Recent …

Paramagnetic centers in biomolecules, such as specific metal ions that are bound to a
protein, affect the nuclei in their surrounding in various ways. One of these effects is the …

Myriad biological processes proceed through states that defy characterization by
conventional atomic-resolution structural biological methods. The invisibility of these …

Pseudocontact shifts in biomolecular NMR using paramagnetic metal tags Page 1 1
Pseudocontact shifts in biomolecular NMR using paramagnetic metal tags Christoph …

Lanthanide ions when complexed by polyamino-polycarboxylate chelators form a class of
compounds of paramount importance in several research and technological areas …

Calmodulin (CaM) is the universal calcium sensor in eukaryotes, regulating the function of
numerous proteins. Crystallography and NMR show that free CaM-4Ca2+ exists in an …

Highlights•Anisotropic paramagnetic effects include PseudoContact Shifts and RDC.•PCS
provide long-range restraints for structure determination.•Practical aspects of PCS structure …

Calcium-loaded calmodulin (CaM/4Ca2+) comprises two domains that undergo rigid body
reorientation from a predominantly extended conformation to a compact one upon binding …

Lanthanoids are gaining popularity as paramagnetic centers for high resolution nuclear
magnetic resonance (NMR) spectroscopy. They provide valuable angular and long-distance …

Determining the three-dimensional structure of a small molecule–protein complex with weak
affinity can be a significant challenge. We present a paramagnetic NMR method to …