CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

The first wave of genetically targeted therapies for cancer focused on drugging gene
products that are recurrently mutated in specific cancer types. However, mutational analysis …

Forward genetic screens are powerful tools for the discovery and functional annotation of
genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short …

Tissue culture of immortal cell strains from diseased patients is an invaluable resource for
medical research but is largely limited to tumor cell lines or transformed derivatives of native …

Gene silencing through sequence-specific targeting of mRNAs by RNAi has enabled
genome-wide functional screens in cultured cells and in vivo in model organisms. These …

5242 2004 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim DOI: 10.1002/anie. 200460437
Angew. Chem. Int. Ed. 2004, 43, 5242–5246 research.[1–11] In particular, the use of CNTs …

Electrical forces for manipulating cells at the microscale include electrophoresis and
dielectrophoresis. Electrophoretic forces arise from the interaction of a cell's charge and an …

Reprogramming of human somatic cells uses readily accessible tissue, such as skin or
blood, to generate embryonic-like induced pluripotent stem cells (iPSCs). This procedure …

Small cell lung cancer (SCLC) is an aggressive lung cancer subtype with extremely poor
prognosis. No targetable genetic driver events have been identified, and the treatment …

Abstract Changes in the interactions among proteins that participate in a biochemical
pathway can reflect the immediate regulatory responses to intrinsic or extrinsic perturbations …