Fluorescence in situ hybridization (FISH) is the gold standard for visualizing genomic DNA in
fixed cells and tissues, but it is incompatible with live-cell imaging, and its combination with …

In-situ detection provides deep insights into the function of genes and their relationship with
diseases by directly visualizing their spatiotemporal behavior. As an emerging in-situ …

The dynamic three-dimensional structures of chromatin and extrachromosomal DNA
molecules regulate fundamental cellular processes and beyond. However, the visualization …

Technologies for gene activation are valuable tools for the study of gene functions and have
a wide range of potential applications in bioengineering and medicine. In contrast to existing …

RNA polymerase I (Pol I) synthesizes about 60% of cellular RNA by transcribing multiple
copies of the ribosomal RNA gene (rDNA). The transcriptional activity of Pol I controls the …

Over the years, our vision of the genome has changed from a linear molecule to that of a
complex 3D structure that follows specific patterns and possesses a hierarchical …

The covalently closed circular DNA (cccDNA) of hepatitis B virus (HBV) is the major obstacle
to curing chronic hepatitis B (CHB). Current cccDNA detection methods are mostly based on …

Maximizing cell survival under stress requires rapid and transient adjustments of RNA and
protein synthesis. However, capturing these dynamic changes at both single-cell level and …

The dynamics of genomic loci pairs and their interactions are essential for transcriptional
regulation and genome organization. However, a robust method for tracking pairwise …

The regulation of gene expression is a fundamental process enabling cells to respond to
internal and external stimuli or to execute developmental programs. Changes in gene …