Differential scanning calorimetry measures the heat capacity of states and the excess heat
associated with transitions that can be induced by temperature change. The integral of the …

Dynamic light scattering (DLS) analyses are routinely used in biology laboratories to detect
aggregates in macromolecular solutions, to determine the size of proteins, nucleic acids …

Standard methods for assessing the thermal stability of viruses can be time consuming and
rather qualitative yet such data is a necessary requisite for vaccine formulation. In this study …

Production of recombinant proteins is a process intensively used in the research laboratory.
In addition, the main biotechnology market products are recombinant proteins and …

Success in determining the three‐dimensional structure of a macromolecule by X‐ray
diffraction methods depends critically on the ability to obtain well ordered crystals of the …

X-ray crystallography is the most commonly employed technique to discern macromolecular
structures, but the crucial step of crystallizing a protein into an ordered lattice amenable to …

Chitin is a homopolymer of β-(1, 4)-linked N-acetyl-D-glucosamine (GlcNAc) and a major
structural component of fungal cell walls. In plants, chitin acts as a microbe-associated …

The great power of protein crystallography to reveal biological structure is often limited by
the tremendous effort required to produce suitable crystals. A hybrid crystal growth predictive …

The preparation of purified, detergent-solubilized membrane proteins in a monodisperse
and stable form is usually a prerequisite for investigation not only of their function but also for …

The preparation of well diffracting crystals is a crucial step of any crystallographic project.
This chapter defines the nature of biomacromolecular crystals, sketches the theoretical …