In eukaryotes, transcription is a discontinuous process with mRNA being generated in
bursts, after the binding of transcription factors (TFs) to regulatory elements on the genome …

The exploitation of low‐affinity molecular interactions in protein labeling is an emerging topic
in optical microscopy. Such non‐covalent and low‐affinity interactions can be realized with …

Abstract 3D super-resolution microscopy with nanometric resolution is a key to fully
complement ultrastructural techniques with fluorescence imaging. Here, we achieve 3D …

Live-cell imaging is a powerful technique to study the dynamics and mechanics of various
biological molecules like proteins, organelles, DNA, and RNA. With the rapid evolution of …

Biomolecular systems, such as proteins, crucially rely on dynamic processes at the
nanoscale. Detecting biomolecular nanodynamics is therefore key to obtaining a …

DNA-PAINT based single-particle tracking (DNA-PAINT-SPT) has recently significantly
enhanced observation times in in vitro SPT experiments by overcoming the constraints of …

Electrochemiluminescence (ECL) imaging, a rapidly evolving technology, has attracted
significant attention in the field of cellular imaging. However, its primary limitation lies in its …

Photobleaching of fluorescent probes limits the observation span of typical single‐molecule
fluorescence measurements and hinders observation of dynamics at long timescales. Here …

Small GTPases play essential roles in the organization of eukaryotic cells. In recent years, it
has become clear that their intracellular functions result from intricate biochemical networks …

Single-molecule localization microscopy (SMLM) has revolutionized light microscopy by
enabling optical resolution down to a few nanometer. Yet, localization precision commonly …