Proteomics by mass spectrometry: approaches, advances, and applications
Mass spectrometry (MS) is the most comprehensive and versatile tool in large-scale
proteomics. In this review, we dissect the overall framework of the MS experiment into its key …
proteomics. In this review, we dissect the overall framework of the MS experiment into its key …
Probing native protein structures by chemical cross-linking, mass spectrometry, and bioinformatics
A Leitner, T Walzthoeni, A Kahraman, F Herzog… - Molecular & Cellular …, 2010 - ASBMB
Chemical cross-linking of reactive groups in native proteins and protein complexes in
combination with the identification of cross-linked sites by mass spectrometry has been in …
combination with the identification of cross-linked sites by mass spectrometry has been in …
Reversed‐phase chromatography with multiple fraction concatenation strategy for proteome profiling of human MCF10A cells
In this study, we evaluated a concatenated low pH (pH 3) and high pH (pH 10) reversed‐
phase liquid chromatography strategy as a first dimension for two‐dimensional liquid …
phase liquid chromatography strategy as a first dimension for two‐dimensional liquid …
Evaluation and optimization of mass spectrometric settings during data-dependent acquisition mode: focus on LTQ-Orbitrap mass analyzers
A Kalli, GT Smith, MJ Sweredoski… - Journal of proteome …, 2013 - ACS Publications
Mass-spectrometry-based proteomics has evolved as the preferred method for the analysis
of complex proteomes. Undoubtedly, recent advances in mass spectrometry instrumentation …
of complex proteomes. Undoubtedly, recent advances in mass spectrometry instrumentation …
Evaluating metabolic stress and plasmid stability in plasmid DNA production by Escherichia coli
F Silva, JA Queiroz, FC Domingues - Biotechnology advances, 2012 - Elsevier
In the context of recombinant DNA technology, the development of feasible and high-
yielding plasmid DNA production processes has regained attention as more evidence for its …
yielding plasmid DNA production processes has regained attention as more evidence for its …
Quantitative proteomics reveals metabolic and pathogenic properties of Chlamydia trachomatis developmental forms
HA Saka, JW Thompson, YS Chen… - Molecular …, 2011 - Wiley Online Library
Chlamydia trachomatis is an obligate intracellular pathogen responsible for ocular and
genital infections of significant public health importance. C. trachomatis undergoes a …
genital infections of significant public health importance. C. trachomatis undergoes a …
Multi-dimensional liquid chromatography in proteomics—a review
Proteomics is the large-scale study of proteins, particularly their expression, structures and
functions. This still-emerging combination of technologies aims to describe and characterize …
functions. This still-emerging combination of technologies aims to describe and characterize …
Analysis of host-cell proteins in biotherapeutic proteins by comprehensive online two-dimensional liquid chromatography/mass spectrometry
C Doneanu, A Xenopoulos, K Fadgen, J Murphy… - MAbs, 2012 - Taylor & Francis
Assays for identification and quantification of host-cell proteins (HCPs) in biotherapeutic
proteins over 5 orders of magnitude in concentration are presented. The HCP assays consist …
proteins over 5 orders of magnitude in concentration are presented. The HCP assays consist …
2DE: the phoenix of proteomics
BM Oliveira, JR Coorssen, D Martins-de-Souza - Journal of proteomics, 2014 - Elsevier
Given the rapid developments in mass spectrometry (MS) in terms of sensitivity, mass
accuracy, and throughput, some have suggested that two-dimensional gel electrophoresis …
accuracy, and throughput, some have suggested that two-dimensional gel electrophoresis …
Salivary exosomal PSMA7: a promising biomarker of inflammatory bowel disease
Inflammatory bowel disease (IBD) is an intestinal immune-dysfunctional disease worldwide
whose prevalence increasing in Asia including China. It is a chronic disease of the …
whose prevalence increasing in Asia including China. It is a chronic disease of the …