Mass spectrometry (MS) is the most comprehensive and versatile tool in large-scale
proteomics. In this review, we dissect the overall framework of the MS experiment into its key …

Chemical cross-linking of reactive groups in native proteins and protein complexes in
combination with the identification of cross-linked sites by mass spectrometry has been in …

In this study, we evaluated a concatenated low pH (pH 3) and high pH (pH 10) reversed‐
phase liquid chromatography strategy as a first dimension for two‐dimensional liquid …

Mass-spectrometry-based proteomics has evolved as the preferred method for the analysis
of complex proteomes. Undoubtedly, recent advances in mass spectrometry instrumentation …

In the context of recombinant DNA technology, the development of feasible and high-
yielding plasmid DNA production processes has regained attention as more evidence for its …

Chlamydia trachomatis is an obligate intracellular pathogen responsible for ocular and
genital infections of significant public health importance. C. trachomatis undergoes a …

Proteomics is the large-scale study of proteins, particularly their expression, structures and
functions. This still-emerging combination of technologies aims to describe and characterize …

Assays for identification and quantification of host-cell proteins (HCPs) in biotherapeutic
proteins over 5 orders of magnitude in concentration are presented. The HCP assays consist …

Given the rapid developments in mass spectrometry (MS) in terms of sensitivity, mass
accuracy, and throughput, some have suggested that two-dimensional gel electrophoresis …

Inflammatory bowel disease (IBD) is an intestinal immune-dysfunctional disease worldwide
whose prevalence increasing in Asia including China. It is a chronic disease of the …