DNA-based points accumulation for imaging in nanoscale topography (DNA-PAINT) is a
powerful super-resolution microscopy method that can acquire high-fidelity images at …

Signaling nanodomains rely on spatial organization of proteins to allow controlled
intracellular signaling. Examples include calcium release sites of cardiomyocytes where …

In recent years, DNA origami nanorulers for superresolution (SR) fluorescence microscopy
have been developed from fundamental proof-of-principle experiments to commercially …

Many commercial as well as custom-built fluorescence microscopes use scientific-grade
cameras that represent a substantial share of the instrument's cost. This holds particularly …

Modern implementations of widefield fluorescence microscopy often rely on sCMOS
cameras, but this camera architecture inherently features pixel-to-pixel variations. Such …

The common assumption that precision is the limit of accuracy in localization microscopy
and the typical absence of comprehensive calibration of optical microscopes lead to a …

Single-molecule localization microscopy enables three-dimensional fluorescence imaging
at tens-of-nanometer resolution, but requires many camera frames to reconstruct a super …

Assessment of the imaging quality in localisation-based super-resolution techniques relies
on an accurate characterisation of the imaging setup and analysis procedures. Test samples …

DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding
of fluorescent DNA 'imagers' to target epitopes. Its performance in biological samples is often …

With promising properties of fast imaging speed, large field-of-view, relative low cost and
many others, back-illuminated sCMOS cameras have been receiving intensive attention for …