The molecular basis for cellular function of intrinsically disordered protein regions

AS Holehouse, BB Kragelund - Nature Reviews Molecular Cell Biology, 2024 - nature.com
Intrinsically disordered protein regions exist in a collection of dynamic interconverting
conformations that lack a stable 3D structure. These regions are structurally heterogeneous …

Small-angle X-ray and neutron scattering

CM Jeffries, J Ilavsky, A Martel, S Hinrichs… - Nature Reviews …, 2021 - nature.com
Small-angle scattering (SAS) is a technique that is able to probe the structural organization
of matter and quantify its response to changes in external conditions. X-ray and neutron …

Physics-driven coarse-grained model for biomolecular phase separation with near-quantitative accuracy

JA Joseph, A Reinhardt, A Aguirre, PY Chew… - Nature Computational …, 2021 - nature.com
Various physics-and data-driven sequence-dependent protein coarse-grained models have
been developed to study biomolecular phase separation and elucidate the dominant …

[HTML][HTML] The SARS-CoV-2 nucleocapsid protein is dynamic, disordered, and phase separates with RNA

J Cubuk, JJ Alston, JJ Incicco, S Singh… - Nature …, 2021 - nature.com
Abstract The SARS-CoV-2 nucleocapsid (N) protein is an abundant RNA-binding protein
critical for viral genome packaging, yet the molecular details that underlie this process are …

Conformational ensembles of the human intrinsically disordered proteome

G Tesei, AI Trolle, N Jonsson, J Betz, FE Knudsen… - Nature, 2024 - nature.com
Intrinsically disordered proteins and regions (collectively, IDRs) are pervasive across
proteomes in all kingdoms of life, help to shape biological functions and are involved in …

Biomolecular phase separation: from molecular driving forces to macroscopic properties

GL Dignon, RB Best, J Mittal - Annual review of physical …, 2020 - annualreviews.org
Biological phase separation is known to be important for cellular organization, which has
recently been extended to a new class of biomolecules that form liquid-like droplets …

Intrinsically disordered regions are poised to act as sensors of cellular chemistry

D Moses, GM Ginell, AS Holehouse… - Trends in biochemical …, 2023 - cell.com
Intrinsically disordered proteins and protein regions (IDRs) are abundant in eukaryotic
proteomes and play a wide variety of essential roles. Instead of folding into a stable …

Sequence determinants of protein phase behavior from a coarse-grained model

GL Dignon, W Zheng, YC Kim, RB Best… - PLoS computational …, 2018 - journals.plos.org
Membraneless organelles important to intracellular compartmentalization have recently
been shown to comprise assemblies of proteins which undergo liquid-liquid phase …

Visualizing the disordered nuclear transport machinery in situ

M Yu, M Heidari, S Mikhaleva, PS Tan, S Mingu… - Nature, 2023 - nature.com
The approximately 120 MDa mammalian nuclear pore complex (NPC) acts as a gatekeeper
for the transport between the nucleus and cytosol. The central channel of the NPC is filled …

PED in 2021: a major update of the protein ensemble database for intrinsically disordered proteins

T Lazar, E Martínez-Pérez, F Quaglia… - Nucleic acids …, 2021 - academic.oup.com
Abstract The Protein Ensemble Database (PED)(https://proteinensemble. org), which holds
structural ensembles of intrinsically disordered proteins (IDPs), has been significantly …