Micronuclei as biomarkers of DNA damage, aneuploidy, inducers of chromosomal hypermutation and as sources of pro-inflammatory DNA in humans

M Fenech, S Knasmueller, C Bolognesi… - … Research/Reviews in …, 2020 - Elsevier
Micronuclei (MNi) are among the most widely studied biomarkers of DNA damage and
chromosomal instability in humans. They originate from chromosome fragments or intact …

The HUman MicroNucleus project on eXfoLiated buccal cells (HUMNXL): The role of life-style, host factors, occupational exposures, health status, and assay protocol

S Bonassi, E Coskun, M Ceppi, C Lando… - … Research/Reviews in …, 2011 - Elsevier
The human buccal micronucleus cytome assay (BMCyt) is one of the most widely used
techniques to measure genetic damage in human population studies. Reducing protocol …

Cytokinesis-block micronucleus cytome assay

M Fenech - Nature protocols, 2007 - nature.com
The cytokinesis-block micronucleus cytome assay is a comprehensive system for measuring
DNA damage, cytostasis and cytotoxicity. DNA damage events are scored specifically in …

HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures

M Fenech, WP Chang, M Kirsch-Volders… - … /Genetic Toxicology and …, 2003 - Elsevier
Criteria for scoring micronuclei and nucleoplasmic bridges in binucleated cells in the
cytokinesis-block micronucleus assay for isolated human lymphocyte cultures are described …

The effect of age, gender, diet and lifestyle on DNA damage measured using micronucleus frequency in human peripheral blood lymphocytes

M Fenech, S Bonassi - Mutagenesis, 2011 - academic.oup.com
Micronucleus (MN) frequency in cytokinesis-blocked peripheral blood lymphocytes (PBL)
has become one of the best-established biomarkers for studying DNA damage occurring in …

Chromosomal changes: induction, detection methods and applicability in human biomonitoring

R Mateuca, N Lombaert, PV Aka, I Decordier… - Biochimie, 2006 - Elsevier
The objective of this state of the art paper is to review the mechanisms of induction, the fate,
the methodology, the sensitivity/specificity and predictivity of two major cytogenetic …

The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure

A Vral, M Fenech, H Thierens - Mutagenesis, 2011 - academic.oup.com
Biological dosimetry, based on the analysis of micronuclei (MN) in the cytokinesis-block
micronucleus (CBMN) assay can be used as an alternative method for scoring dicentric …

The in vitro MN assay in 2011: origin and fate, biological significance, protocols, high throughput methodologies and toxicological relevance

M Kirsch-Volders, G Plas, A Elhajouji… - Archives of …, 2011 - Springer
Micronuclei (MN) are small, extranuclear bodies that arise in dividing cells from acentric
chromosome/chromatid fragments or whole chromosomes/chromatids lagging behind in …

[HTML][HTML] Micronuclei and genome chaos: changing the system inheritance

CJ Ye, Z Sharpe, S Alemara, S Mackenzie, G Liu… - Genes, 2019 - mdpi.com
Micronuclei research has regained its popularity due to the realization that genome chaos, a
rapid and massive genome re-organization under stress, represents a major common …

[HTML][HTML] The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and …

M Milić, M Ceppi, M Bruzzone, A Azqueta… - … Research/Reviews in …, 2021 - Elsevier
The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular
methods for assessing DNA damage in human population. One of the open issues …