Pre-mRNA maturation frequently occurs at the same time and place as transcription by RNA
polymerase II. The co-transcriptionality of mRNA processing has permitted the evolution of …

Highlights•Dynamic changes in the nucleosome structure take place when RNA polymerase
II transcribes through a nucleosome.•Nucleosome reassembly may be mediated by the …

During gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin,
but the mechanism has remained elusive. Using cryo–electron microscopy, we obtained …

During gene transcription, RNA polymerase II (RNA Pol II) passes nucleosomes with the
help of various elongation factors. Here, we show that RNA Pol II achieves efficient …

Nucleosomes are elementary building blocks of chromatin in eukaryotes. They tightly
wrap∼ 147 DNA base pairs around an octamer of histone proteins. How nucleosome …

Despite the well-established role of nuclear organization in the regulation of gene
expression, little is known about the reverse: how transcription shapes the spatial …

Sophisticated gene-regulatory mechanisms probably evolved in prokaryotes billions of
years before the emergence of modern eukaryotes, which inherited the same basic …

In eukaryotes, all genetic processes take place in the cell nucleus, where DNA is packaged
as chromatin in'beads-on-a-string'nucleosome arrays. RNA polymerase II (RNAPII) …

DNA accessibility to regulatory proteins is substantially influenced by nucleosome structure
and dynamics. The facilitates chromatin transcription (FACT) complex increases the …

The transcription elongation factor Spt5 is conserved from bacteria to humans. In
eukaryotes, Spt5 forms a complex with Spt4 and regulates processive transcription …