The emerging inverse electron demand Diels–Alder (IEDDA) reaction stands out from other
bioorthogonal reactions by virtue of its unmatchable kinetics, excellent orthogonality and …

Over 500 natural and synthetic amino acids have been genetically encoded in the last two
decades. Incorporating these noncanonical amino acids into proteins enables many …

Bioorthogonal chemistries enable researchers to interrogate biomolecules in living systems.
These reactions are highly selective and biocompatible, and can be performed in many …

Our understanding of the complex molecular processes of living organisms at the molecular
level is growing exponentially. This knowledge, together with a powerful arsenal of tools for …

Over the past 16 years, genetic code expansion and reprogramming in living organisms has
been transformed by advances that leverage the unique properties of pyrrolysyl-tRNA …

Monosubstituted tetrazines are important bioorthogonal reactive tools due to their rapid
ligation with trans-cyclooctene. However, their application is limited by the reactivity–stability …

Glycopolymers are synthetic macromolecules having pendant sugar moieties and widely
utilized to target cancer cells. They are usually considered as a hydrophilic segment of …

Using an engineered pyrrolysyl-tRNA synthetase mutant together with tRNACUAPyl, we
have genetically encoded Nε-(7-azidoheptanoyl)-l-lysine (AzHeK) by amber codon in …

As a member of a highly conserved family of NAD+-dependent histone deacetylases, Sirt6 is
a key regulator of mammalian genome stability, metabolism, and life span. Previous studies …

Bioorthogonal reactions are widely used for the chemical modification of biomolecules. The
application of vinylboronic acids (VBAs) as non‐strained, synthetically accessible and water …