Phage therapy: what have we learned?

A Górski, R Międzybrodzki, M Łobocka… - Viruses, 2018 - mdpi.com
In this article we explain how current events in the field of phage therapy may positively
influence its future development. We discuss the shift in position of the authorities …

Whole-genome sequencing in outbreak analysis

CA Gilchrist, SD Turner, MF Riley… - Clinical microbiology …, 2015 - Am Soc Microbiol
In addition to the ever-present concern of medical professionals about epidemics of
infectious diseases, the relative ease of access and low cost of obtaining, producing, and …

Increased production of L-serine in Escherichia coli through adaptive laboratory evolution

H Mundhada, JM Seoane, K Schneider, A Koza… - Metabolic …, 2017 - Elsevier
L-serine is a promising building block biochemical with a high theoretical production yield
from glucose. Toxicity of L-serine is however prohibitive for high-titer production in E. coli …

Effect of deletion of genes involved in lipopolysaccharide core and O-antigen synthesis on virulence and immunogenicity of Salmonella enterica serovar Typhimurium

Q Kong, J Yang, Q Liu, P Alamuri, KL Roland… - Infection and …, 2011 - Am Soc Microbiol
Lipopolysaccharide (LPS) is a major virulence factor of Salmonella enterica serovar
Typhimurium and is composed of lipid A, core oligosaccharide (C-OS), and O-antigen …

ORBIT: a new paradigm for genetic engineering of mycobacterial chromosomes

KC Murphy, SJ Nelson, S Nambi… - MBio, 2018 - Am Soc Microbiol
Two efficient recombination systems were combined to produce a versatile method for
chromosomal engineering that obviates the need to prepare double-stranded DNA (dsDNA) …

λ recombination and recombineering

KC Murphy - EcoSal Plus, 2016 - Am Soc Microbiol
The bacteriophage λ Red homologous recombination system has been studied over the
past 50 years as a model system to define the mechanistic details of how organisms …

Bacterial genetic engineering by means of recombineering for reverse genetics

U Fels, K Gevaert, P Van Damme - Frontiers in Microbiology, 2020 - frontiersin.org
Serving a robust platform for reverse genetics enabling the in vivo study of gene functions
primarily in enterobacteriaceae, recombineering-or recombination-mediated genetic …

A CRISPR-assisted nonhomologous end-joining strategy for efficient genome editing in Mycobacterium tuberculosis

MY Yan, SS Li, XY Ding, XP Guo, Q Jin, YC Sun - MBio, 2020 - Am Soc Microbiol
New tools for genetic manipulation of Mycobacterium tuberculosis are needed for the
development of new drug regimens and vaccines aimed at curing tuberculosis infections …

Rapid and Highly Efficient Method for Scarless Mutagenesis within the Salmonella enterica Chromosome

K Blank, M Hensel, RG Gerlach - PloS one, 2011 - journals.plos.org
Direct manipulation of bacterial chromosomes by recombination-based techniques has
become increasingly important for both cognitive and applied research. Here we …

Bacterial cellular engineering by genome editing and gene silencing

N Nakashima, K Miyazaki - International journal of molecular sciences, 2014 - mdpi.com
Genome editing is an important technology for bacterial cellular engineering, which is
commonly conducted by homologous recombination-based procedures, including gene …