Planarians are members of the Platyhelminthes (flatworms). These animals have evolved a
remarkable stem cell system. A single pluripotent adult stem cell type (“neoblast”) gives rise …

RNA-fluorescence in situ hybridization (FISH) is a powerful tool to visualize target
messenger RNA transcripts in cultured cells, tissue sections or whole-mount preparations …

INTRODUCTION The ability to regenerate tissues lost to damage or disease is widely but
nonuniformly distributed in vertebrates. Some animals such as teleost fishes can regenerate …

Proliferating cells known as neoblasts include pluripotent stem cells (PSCs) that sustain
tissue homeostasis and regeneration of lost body parts in planarians. However, the lack of …

Planarians are flatworms capable of regenerating any missing body region. This capacity is
mediated by neoblasts, a proliferative cell population that contains pluripotent stem cells …

Abstract Background The freshwater planarian Schmidtea mediterranea has emerged as a
powerful model for studies of regenerative, stem cell, and germ cell biology. Whole-mount in …

Regeneration requires signaling from a wound site for detection of the wound and a
mechanism that determines the nature of the injury to specify the appropriate regenerative …

Regeneration starts with injury. Yet how injuries affect gene expression in different cell types
and how distinct injuries differ in gene expression remain unclear. We defined the …

Many long-lived organisms, including humans, can regenerate some adult tissues lost to
physical injury or disease. Much of the previous research on mechanisms of regeneration …

Regeneration requires both potential and instructions for tissue replacement. In planarians,
pluripotent stem cells have the potential to produce all new tissue. The identities of the cells …