Miniaturized chemical tags for optical imaging

S Benson, F De Moliner, W Tipping… - Angewandte Chemie …, 2022 - Wiley Online Library
Recent advances in optical bioimaging have prompted the need for minimal chemical
reporters that can retain the molecular recognition properties and activity profiles of …

The role of Raman spectroscopy within quantitative metabolomics

C Lima, H Muhamadali… - Annual Review of …, 2021 - annualreviews.org
Ninety-four years have passed since the discovery of the Raman effect, and there are
currently more than 25 different types of Raman-based techniques. The past two decades …

Super-resolution SRS microscopy with A-PoD

H Jang, Y Li, AA Fung, P Bagheri, K Hoang… - Nature …, 2023 - nature.com
Stimulated Raman scattering (SRS) offers the ability to image metabolic dynamics with high
signal-to-noise ratio. However, its spatial resolution is limited by the numerical aperture of …

Phase‐separated multienzyme compartmentalization for terpene biosynthesis in a prokaryote

Y Wang, M Liu, Q Wei, W Wu, Y He, J Gao… - Angewandte …, 2022 - Wiley Online Library
Liquid–liquid phase separation (LLPS) forms biomolecular condensates or coacervates in
cells. Metabolic enzymes can form phase‐separated subcellular compartments that enrich …

Quantitative stimulated Raman scattering microscopy: promises and pitfalls

B Manifold, D Fu - Annual Review of Analytical Chemistry, 2022 - annualreviews.org
Since its first demonstration, stimulated Raman scattering (SRS) microscopy has become a
powerful chemical imaging tool that shows promise in numerous biological and biomedical …

A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems

S Bakthavatsalam, K Dodo, M Sodeoka - RSC Chemical Biology, 2021 - pubs.rsc.org
Alkyne functional groups have Raman signatures in a region (1800 cm− 1 to 2800 cm− 1)
that is free from interference from cell components, known as the “silent region”, and alkyne …

Long-term in vivo imaging of mouse spinal cord through an optically cleared intervertebral window

W Wu, S He, J Wu, C Chen, X Li, K Liu, JY Qu - Nature communications, 2022 - nature.com
The spinal cord accounts for the main communication pathway between the brain and the
peripheral nervous system. Spinal cord injury is a devastating and largely irreversible …

Toward Raman subcellular imaging of endothelial dysfunction

A Adamczyk, E Matuszyk, B Radwan… - Journal of Medicinal …, 2021 - ACS Publications
Multiple diseases are at some point associated with altered endothelial function, and
endothelial dysfunction (ED) contributes to their pathophysiology. Biochemical changes of …

Spectroscopic coherent Raman imaging of Caenorhabditis elegans reveals lipid particle diversity

WW Chen, GA Lemieux, CH Camp Jr… - Nature chemical …, 2020 - nature.com
Caenorhabditis elegans serves as a model for understanding adiposity and its connections
to aging. Current methodologies do not distinguish between fats serving the energy needs of …

Imaging sub-cellular methionine and insulin interplay in triple negative breast cancer lipid droplet metabolism

AA Fung, K Hoang, H Zha, D Chen, W Zhang… - Frontiers in …, 2022 - frontiersin.org
Triple negative breast cancer (TNBC) is a particularly aggressive cancer subtype that is
difficult to diagnose due to its discriminating epidemiology and obscure metabolome. For the …