With both catalytic and genetic functions, ribonucleic acid (RNA) is perhaps the most
pluripotent chemical species in molecular biology, and its functions are intimately linked to …

Biological macromolecules are highly flexible and continually undergo conformational
fluctuations on a broad spectrum of timescales. It has long been recognized that dynamics …

Structural determination of N-and O-linked glycans as well as polysaccharides is hampered
by the limited spectral dispersion. The computerized approach CASPER, an acronym for …

We present a brief overview of explicit solvent molecular dynamics (MD) simulations of
nucleic acids. We explain physical chemistry limitations of the simulations, namely, the …

Many recently discovered noncoding RNAs do not fold into a single native conformation but
sample many different conformations along their free-energy landscape to carry out their …

The ability to modulate alignment and measure multiple independent sets of NMR residual
dipolar couplings (RDCs) has made it possible to characterize internal motions in proteins at …

We describe a strategy for constructing atomic resolution dynamical ensembles of RNA
molecules, spanning up to millisecond timescales, that combines molecular dynamics (MD) …

Ribonucleic acid structure determination by NMR spectroscopy relies primarily on local
structural restraints provided by 1 H–1 H NOEs and J-couplings. When employed loosely …

This protocol describes an efficient method for the preparation of riboswitch domains
comprising up to∼ 200 nt containing site-specific nucleoside modifications. The strategy is …

The 5′-untranslated regions of all gammaretroviruses contain a conserved “double-hairpin
motif”(ΨCD) that is required for genome packaging. Both hairpins (SL-C and SL-D) contain …