Paving the way to single-molecule protein sequencing

L Restrepo-Pérez, C Joo, C Dekker - Nature nanotechnology, 2018 - nature.com
Proteins are major building blocks of life. The protein content of a cell and an organism
provides key information for the understanding of biological processes and disease. Despite …

Droplet-based microfluidics in biomedical applications

L Amirifar, M Besanjideh, R Nasiri, A Shamloo… - …, 2022 - iopscience.iop.org
Droplet-based microfluidic systems have been employed to manipulate discrete fluid
volumes with immiscible phases. Creating the fluid droplets at microscale has led to a …

Microfluidic single‐cell omics analysis

X Xu, J Wang, L Wu, J Guo, Y Song, T Tian, W Wang… - Small, 2020 - Wiley Online Library
The commonly existing cellular heterogeneity plays a critical role in biological processes
such as embryonic development, cell differentiation, and disease progress. Single‐cell …

Microtechnologies for single-cell and spatial multi-omics

Y Deng, Z Bai, R Fan - Nature Reviews Bioengineering, 2023 - nature.com
Single-cell omics assays allow the identification of the type, subtype and functional state of a
single cell. To put such single-cell data in the context of tissues, spatially resolved omics can …

[HTML][HTML] Circulating tumor cells in hepatocellular carcinoma: single-cell based analysis, preclinical models, and clinical applications

Q Zhang, Y Rong, K Yi, L Huang, M Chen, F Wang - Theranostics, 2020 - ncbi.nlm.nih.gov
Circulating tumor cells (CTCs) are shed into the bloodstream from primary tumors and
metastatic lesions and provide significant information about tumor progression and …

Label‐free optofluidic nanobiosensor enables real‐time analysis of single‐cell cytokine secretion

X Li, M Soler, C Szydzik, K Khoshmanesh, J Schmidt… - Small, 2018 - Wiley Online Library
Single‐cell analysis of cytokine secretion is essential to understand the heterogeneity of
cellular functionalities and develop novel therapies for multiple diseases. Unraveling the …

Microfluidics towards single cell resolution protein analysis

P Chen, D Chen, S Li, X Ou, BF Liu - TrAC Trends in Analytical Chemistry, 2019 - Elsevier
Single cell analysis has aroused great interest with its remarkable ability to investigate cell-
to-cell heterogeneity in large populations. However, probing protein information at single …

A novel approach to design and fabricate an electrothermal microgripper for cell manipulation

M Akbari, F Barazandeh, H Barati - Sensors and Actuators A: Physical, 2022 - Elsevier
Microgrippers are used in cell manipulation, micro-assembly, and material characterization.
However, rarely a systematic approach is presented for microgripper design. The current …

Advancing single-cell proteomics and metabolomics with microfluidic technologies

Y Liu, X Chen, Y Zhang, J Liu - Analyst, 2019 - pubs.rsc.org
Recent advances in single-cell analysis have unraveled substantial heterogeneity among
seemingly identical cells at genomic and transcriptomic levels. These discoveries have …

Mass spectrometry‐based strategies for single‐cell metabolomics

R Hu, Y Li, Y Yang, M Liu - Mass Spectrometry Reviews, 2023 - Wiley Online Library
Single cell analysis has drawn increasing interest from the research community due to its
capability to interrogate cellular heterogeneity, allowing refined tissue classification and …