Examining horizontal gene transfer in microbial communities

IL Brito - Nature Reviews Microbiology, 2021 - nature.com
Bacteria acquire novel DNA through horizontal gene transfer (HGT), a process that enables
an organism to rapidly adapt to changing environmental conditions, provides a competitive …

Electroporation-based applications in biotechnology

T Kotnik, W Frey, M Sack, SH Meglič, M Peterka… - Trends in …, 2015 - cell.com
Electroporation is already an established technique in several areas of medicine, but many
of its biotechnological applications have only started to emerge; we review here some of the …

Acquired antibiotic resistance genes: an overview

AHAM Van Hoek, D Mevius, B Guerra… - Frontiers in …, 2011 - frontiersin.org
In this review an overview is given on antibiotic resistance (AR) mechanisms with special
attentions to the AR genes described so far preceded by a short introduction on the …

Engineered integrative and conjugative elements for efficient and inducible DNA transfer to undomesticated bacteria

JAN Brophy, AJ Triassi, BL Adams, RL Renberg… - Nature …, 2018 - nature.com
Engineering microorganisms to promote human or plant health will require manipulation of
robust bacteria that are capable of surviving in harsh, competitive environments. Genetic …

Fluorescence in situ hybridization (FISH) and cell sorting of living bacteria

G Batani, K Bayer, J Böge, U Hentschel, T Thomas - Scientific reports, 2019 - nature.com
Despite the development of several cultivation methods, the rate of discovery of
microorganisms that are yet-to-be cultivated outpaces the rate of isolating and cultivating …

A genetic system for Clostridium ljungdahlii: a chassis for autotrophic production of biocommodities and a model homoacetogen

C Leang, T Ueki, KP Nevin… - Applied and environmental …, 2013 - Am Soc Microbiol
Methods for genetic manipulation of Clostridium ljungdahlii are of interest because of the
potential for production of fuels and other biocommodities from carbon dioxide via microbial …

[HTML][HTML] Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment

M Zhu, MW Frank, CD Radka, S Jeanfavre, J Xu… - Cell, 2024 - cell.com
Bacterial vaginosis (BV), a common syndrome characterized by Lactobacillus-deficient
vaginal microbiota, is associated with adverse health outcomes. BV often recurs after …

Microbial base editing: a powerful emerging technology for microbial genome engineering

Y Wang, Y Liu, P Zheng, J Sun, M Wang - Trends in Biotechnology, 2021 - cell.com
Genome engineering is crucial for answering fundamental questions about, and exploring
practical applications of, microorganisms. Various microbial genome-engineering tools …

Nanowire-modified three-dimensional electrode enabling low-voltage electroporation for water disinfection

ZY Huo, X Xie, T Yu, Y Lu, C Feng… - Environmental science & …, 2016 - ACS Publications
More than 10% of the people in the world still suffer from inadequate access to clean water.
Traditional water disinfection methods (eg, chlorination and ultraviolet radiation) include …

CRISPR-Cas12a-Mediated Gene Deletion and Regulation in Clostridium ljungdahlii and Its Application in Carbon Flux Redirection in Synthesis Gas Fermentation

R Zhao, Y Liu, H Zhang, C Chai, J Wang… - ACS Synthetic …, 2019 - ACS Publications
Uncovering the full potential of gas-fermenting Clostridia, attractive autotrophic bacteria
capable of using synthesis gases (CO–CO2–H2) to produce a range of chemicals and fuels …