Since at least the last common ancestor of all life on Earth, genetic information has been
stored in a four-letter alphabet that is propagated and retrieved by the formation of two base …

Gram-negative bacteria release Outer Membrane Vesicles (OMVs) into the extracellular
environment. Recent studies recognized these vesicles as vectors to horizontal gene …

In this review, the basic concepts and applications of design of experiments (DoE) in
recombinant protein biotechnology will be discussed. The production of recombinant …

The capture of metagenomic DNA in large clone libraries provides the opportunity to study
microbial diversity that is inaccessible using culture-dependent methods. In this study, we …

The uptake of exogenous DNA materials through the cell membrane by bacteria, known as
transformation, is essential for the genetic manipulation of bacteria and, thus, plays key roles …

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most threatening
pathogens due to its multi-drug resistance (MDR) and strong biofilm-forming capacity. Here …

Bacterial transformation is a crucial part of cloning process and has been widely used in
many studies (Swords, 2003; Gigova et al., 2006). The mechanism is marked by two phases …

Background Aberrant glycosylation patterns play a crucial role in the development of cancer
cells as they promote tumor growth and aggressiveness. Lectins recognize carbohydrate …

The antimicrobial resistance (AMR) crisis urgently requires countermeasures for reducing
the dissemination of plasmid-borne resistance genes. Of particular concern are opportunistic …

Peracetic acid (PAA) is an emerging disinfectant with a low disinfection by-product formation
potential, but how PAA destroys gene function after killing bacteria remains to be studied …