Engineering Ag43 signal peptides with bacterial display and selection

DWS Koh, JH Tay, SKE Gan - Methods and Protocols, 2022 - mdpi.com
Protein display, secretion, and export in prokaryotes are essential for utilizing microbial
systems as engineered living materials, medicines, biocatalysts, and protein factories. To …

Variable-heavy (VH) families influencing IgA1&2 engagement to the antigen, FcαRI and superantigen proteins G, A, and L

WL Ling, CTT Su, WH Lua, JY Yeo, JJ Poh, YL Ng… - Scientific Reports, 2022 - nature.com
Interest in IgA as an alternative antibody format has increased over the years with much
remaining to be investigated in relation to interactions with immune cells. Considering the …

The influence of variable-heavy chain families on IgG2, 3, 4, FcγRs and B-cell superantigens protein G and L binding using biolayer interferometry

AM Deacy, SKE Gan - Antibody Therapeutics, 2023 - academic.oup.com
As the most abundant immunoglobulin in blood and the most common human isotype used
for therapeutic monoclonal antibodies, the engagement and activation of its Fc receptors by …

[HTML][HTML] The synergistic effects of the constant region and variable heavy chain families of multimeric immunoglobulin M on its interaction with Fc-mu receptor and …

WL Ling, SKE Gan - Exploration of Immunology, 2022 - explorationpub.com
Aim: As the primary response antibody with increasing use as a therapeutic immunoglobulin
(Ig) format, IgM is also the largest antibody structure among the five major human isotypes …

The synergistic effects of the VH families and CH regions of multimeric IgM on its interaction with FcμR, and antigen

WL Ling, SKE Gan - bioRxiv, 2022 - biorxiv.org
As the primary response antibody with increasing interest as a therapeutic antibody format,
IgM is also the largest antibody structure among the five major human isotypes …

Unravelling Antibody-Induced Mechanical Stability of Antigen: Insights from Single-Molecule Studies

S Haldar, S Chakrabarty, S chakraborty, S Pandit… - bioRxiv, 2024 - biorxiv.org
The intricate nature of antigen-antibody interactions plays a pivotal role in immunological
responses. Despite the multitude of ligand-binding sites on antigens, the influence of …

The Influence of Variable-Heavy (VH) Chain Families on IgG2, 3, 4 on FcγRs and Antibody Superantigens Protein G and L Binding using Biolayer Interferometry

AM Deacy, SKE Gan - bioRxiv, 2023 - biorxiv.org
Background As the most abundant immunoglobulin in blood and the most common human
isotype used for therapeutic monoclonal antibodies, the engagement and subsequent …

Augmenting the Signal Peptide of the Ag43 Autotransporter for the improved heterologous display of sfGFP using Fluorescence-Activated Cell Sorting (FACs)-assisted …

DWS Koh, JH Tay, SKE Gan - bioRxiv, 2022 - biorxiv.org
Protein display, secretion and export in prokaryotes are essential for utilizing microbial
systems as engineered living materials for medicines, biocatalysts, and protein factories. To …