Microbial proteases applications

A Razzaq, S Shamsi, A Ali, Q Ali, M Sajjad… - … in bioengineering and …, 2019 - frontiersin.org
The use of chemicals around the globe in different industries has increased tremendously,
affecting the health of people. The modern world intends to replace these noxious chemicals …

Dynamic regulation of the 26S proteasome: from synthesis to degradation

RS Marshall, RD Vierstra - Frontiers in Molecular Biosciences, 2019 - frontiersin.org
All eukaryotes rely on selective proteolysis to control the abundance of key regulatory
proteins and maintain a healthy and properly functioning proteome. Most of this turnover is …

The necessity of and strategies for improving confidence in the accuracy of western blots

R Ghosh, JE Gilda, AV Gomes - Expert review of proteomics, 2014 - Taylor & Francis
Western blotting is one of the most commonly used laboratory techniques for identifying
proteins and semi-quantifying protein amounts; however, several recent findings suggest …

[HTML][HTML] Regulation of proteasome activity in health and disease

M Schmidt, D Finley - Biochimica et Biophysica Acta (BBA)-Molecular Cell …, 2014 - Elsevier
The ubiquitin–proteasome system (UPS) is the primary selective degradation system in the
nuclei and cytoplasm of eukaryotic cells, required for the turnover of myriad soluble proteins …

Oxidative stress-mediated regulation of proteasome complexes

CT Aiken, RM Kaake, X Wang, L Huang - Molecular & Cellular Proteomics, 2011 - ASBMB
Oxidative stress has been implicated in aging and many human diseases, notably
neurodegenerative disorders and various cancers. The reactive oxygen species that are …

Structure, dynamics and function of the 26S proteasome

Y Mao - Macromolecular protein complexes III: structure and …, 2021 - Springer
The 26S proteasome is the most complex ATP-dependent protease machinery, of~ 2.5 MDa
mass, ubiquitously found in all eukaryotes. It selectively degrades ubiquitin-conjugated …

[HTML][HTML] Age-related deficits in skeletal muscle recovery following disuse are associated with neuromuscular junction instability and ER stress, not impaired protein …

LM Baehr, DWD West, G Marcotte, AG Marshall… - Aging (Albany …, 2016 - ncbi.nlm.nih.gov
Age-related loss of muscle mass and strength can be accelerated by impaired recovery of
muscle mass following a transient atrophic stimulus. The aim of this study was to identify the …

Ubiquitin and ubiquitin-like proteins in protein regulation

J Herrmann, LO Lerman, A Lerman - Circulation research, 2007 - Am Heart Assoc
The discovery of the ubiquitin system was awarded with the Nobel Prize in Chemistry in
2004. Labeling of intracellular proteins for degradation by a multienzymatic complex, called …

Regulation of the 26S Proteasome Complex During Oxidative Stress

X Wang, J Yen, P Kaiser, L Huang - Science signaling, 2010 - science.org
The proteasome plays a pivotal role in the cellular response to oxidative stress. Here, we
used biochemical and mass spectrometric methods to investigate structural changes in the …

Muscle sparing in muscle RING finger 1 null mice: response to synthetic glucocorticoids

LM Baehr, JD Furlow, SC Bodine - The Journal of physiology, 2011 - Wiley Online Library
Non‐Technical Summary Skeletal muscle has the capacity to modify its size in response to
external cues such as mechanical load, neural activity, hormones, stress and nutritional …