The last few decades have led to an explosion in our understanding of the major roles that
small regulatory RNAs (sRNAs) play in regulatory circuits and the responses to stress in …

Bacteria are an exceedingly diverse group of organisms whose molecular exploration is
experiencing a renaissance. While the classical view of bacterial gene expression was …

Base pairing RNAs modulate gene expression in all studied organisms. In many bacteria,
the base pairing between most small regulatory RNAs (sRNAs) and their targets is mediated …

The functional annotation of transcriptomes and identification of noncoding RNA (ncRNA)
classes has been greatly facilitated by the advent of next-generation RNA sequencing …

The molecular roles of many RNA‐binding proteins in bacterial post‐transcriptional gene
regulation are not well understood. Approaches combining in vivo UV crosslinking with RNA …

Understanding RNA processing and turnover requires knowledge of cleavages by major
endoribonucleases within a living cell. We have employed TIER-seq (transiently inactivating …

Small noncoding RNAs (sRNAs) are crucial regulators of gene expression in bacteria.
Acting in concert with major RNA chaperones such as Hfq or ProQ, sRNAs base-pair with …

Over the past two decades, small noncoding RNAs (sRNAs) that regulate mRNAs by short
base pairing have gone from a curiosity to a major class of post-transcriptional regulators in …

Highlights•Novel cross-linking and sequencing methods uncover new sRNAs and sRNA
networks.•Many sRNAs are encoded within the 3′ and 5′ UTRs of mRNA as well as …

RNA is involved in the regulation of multiple cellular processes, often by forming sequence-
specific base pairs with cellular RNA or DNA targets that must be identified among the large …