Identifying function-enhancing enzyme variants is a 'holy grail'challenge in protein science
because it will allow researchers to expand the biocatalytic toolbox for late-stage …

Protein engineering holds immense promise in shaping the future of biomedicine and
biotechnology. This Review focuses on our ongoing development of Mutexa, a …

Directed evolution facilitates enzyme engineering via iterative rounds of mutagenesis.
Despite the wide applications of high-throughput screening, building “smart libraries” to …

Substrate positioning dynamics (SPD) orients the substrate in the active site, thereby
influencing catalytic efficiency. However, it remains unknown whether SPD effects originate …

Nitropolycyclic aromatic hydrocarbons (NPAHs) present one of the most important airborne
pollutants. Recent studies have shown that one of the most abundant NPAHs, 2 …

Abstract Polycyclic Aromatic Hydrocarbon (PAHs) presents one of the most abundant class
of environmental pollutants. Recent study shows a lab-synthesized PAHs derivative …

Lasso peptides are a subclass of ribosomally synthesized and post-translationally modified
peptides with a slipknot conformation. With superior thermal stability, protease resistance …

Flavoprotein monooxygenases catalyze reactions, including hydroxylation and epoxidation,
involved in the catabolism, detoxification, and biosynthesis of natural substrates and …

Due to the emergence of antibiotic resistance, the need to explore novel antibiotics and/or
novel strategies to counter antibiotic resistance is of utmost importance. In this work, we …

Data-driven modeling has emerged as a new paradigm for biocatalyst design and discovery.
Biocatalytic databases that integrate enzyme structure and function data are in urgent need …