The rise in fluorescence-based imaging techniques over the past 3 decades has improved
the ability of researchers to scrutinize live cell biology at increased spatial and temporal …

Protein diffusion is crucial for understanding the formation of protein complexes in vivo and
has been the subject of many fluorescence microscopy studies in cells; however, such …

Understanding biological function requires the identification and characterization of complex
patterns of molecules. Single-molecule localization microscopy (SMLM) can quantitatively …

Atomic force microscopy (AFM) provides an effective, label-free technique enabling the
imaging of live bacteria under physiological conditions with nanometre precision. However …

Bacteria as single-cell organisms are important model systems to study cellular mechanisms
and functions. In recent years and with the help of advanced fluorescence microscopy …

Single-particle tracking is an important technique in the life sciences to understand the
kinetics of biomolecules. The analysis of apparent diffusion coefficients in vivo, for example …

Single-molecule fluorescence microscopy studies of bacteria provide unique insights into
the mechanisms of cellular processes and protein machineries in ways that are unrivalled by …

Labeling of bacterial cells with fluorescent proteins allows tracking the bacteria in
competition and interactomic in vivo and in vitro studies. During the last years, a few plasmid …

In this review, we discuss multi-color single-molecule imaging and tracking strategies for
studying microbial cell biology. We first summarize and compare the methods in a detailed …

The different niches through which bacteria move during their life cycle require a fast
response to the many environmental queues they encounter. The sensing of these stimuli …