Fluorescent proteins as biomarkers and biosensors: throwing color lights on molecular and cellular processes

OV Stepanenko, VV Verkhusha… - Current Protein and …, 2008 - ingentaconnect.com
Green fluorescent protein (GFP) from jellyfish Aequorea victoria is the most extensively
studied and widely used in cell biology protein. GFP-like proteins constitute a fast growing …

Chemically stable fluorescent proteins for advanced microscopy

BC Campbell, MG Paez-Segala, LL Looger… - Nature …, 2022 - nature.com
We report the rational engineering of a remarkably stable yellow fluorescent protein
(YFP),'hyperfolder YFP'(hfYFP), that withstands chaotropic conditions that denature most …

Beta-barrel scaffold of fluorescent proteins: folding, stability and role in chromophore formation

OV Stepanenko, OV Stepanenko, IM Kuznetsova… - International review of …, 2013 - Elsevier
This review focuses on the current view of the interaction between the β-barrel scaffold of
fluorescent proteins and their unique chromophore located in the internal helix. The …

Engineering and characterization of a superfolder green fluorescent protein

JD Pédelacq, S Cabantous, T Tran, TC Terwilliger… - Nature …, 2006 - nature.com
Existing variants of green fluorescent protein (GFP) often misfold when expressed as fusions
with other proteins. We have generated a robustly folded version of GFP …

Fluorescent protein lifetimes report densities and phases of nuclear condensates during embryonic stem-cell differentiation

K Joron, JO Viegas, L Haas-Neill, S Bier, P Drori… - Nature …, 2023 - nature.com
Fluorescent proteins (FP) are frequently used for studying proteins inside cells. In advanced
fluorescence microscopy, FPs can report on additional intracellular variables. One variable …

Selective magnetic nanoheating: Combining iron oxide nanoparticles for multi-hot-spot induction and sequential regulation

JG Ovejero, I Armenia, D Serantes… - Nano …, 2021 - ACS Publications
The contactless heating capacity of magnetic nanoparticles (MNPs) has been exploited in
fields such as hyperthermia cancer therapy, catalysis, and enzymatic thermal regulation …

GFP-like proteins stably accumulate in lysosomes

H Katayama, A Yamamoto, N Mizushima… - Cell structure and …, 2008 - jstage.jst.go.jp
GFPs), and more recently the novel GFP-like proteins from Anthozoa have greatly advanced
our technologies for fluorescently labeling cells, organelles, and proteins. It has been …

Crystal structure of enhanced green fluorescent protein to 1.35 Å resolution reveals alternative conformations for Glu222

JAJ Arpino, PJ Rizkallah, DD Jones - 2012 - journals.plos.org
Enhanced Green Fluorescent Protein (EGFP) is one of the most widely used engineered
variants of the original wild-type Green Fluorescent Protein. Here, we report the high …

Real-time imaging of discrete exocytic events mediating surface delivery of AMPA receptors

GA Yudowski, MA Puthenveedu… - Journal of …, 2007 - Soc Neuroscience
We directly resolved discrete exocytic fusion events mediating insertion of AMPA-type
glutamate receptors (AMPARs) to the somatodendritic surface of rat hippocampal pyramidal …

Sweeping away protein aggregation with entropic bristles: intrinsically disordered protein fusions enhance soluble expression

AA Santner, CH Croy, FH Vasanwala, VN Uversky… - Biochemistry, 2012 - ACS Publications
Intrinsically disordered, highly charged protein sequences act as entropic bristles (EBs),
which, when translationally fused to partner proteins, serve as effective solubilizers by …