[HTML][HTML] CLIP and complementary methods

M Hafner, M Katsantoni, T Köster, J Marks… - Nature Reviews …, 2021 - nature.com
RNA molecules start assembling into ribonucleoprotein (RNP) complexes during
transcription. Dynamic RNP assembly, largely directed by cis-acting elements on the RNA …

Analysis of transcriptome and epitranscriptome in plants using PacBio Iso-Seq and nanopore-based direct RNA sequencing

L Zhao, H Zhang, MV Kohnen, KVSK Prasad… - Frontiers in …, 2019 - frontiersin.org
Nanopore sequencing from Oxford Nanopore Technologies (ONT) and Pacific BioSciences
(PacBio) single-molecule real-time (SMRT) long-read isoform sequencing (Iso-Seq) are …

Liquid-liquid phase separation of RBGD2/4 is required for heat stress resistance in Arabidopsis

S Zhu, J Gu, J Yao, Y Li, Z Zhang, W Xia, Z Wang… - Developmental Cell, 2022 - cell.com
As sessile organisms, plants are highly sensitive to environmental stresses. In response to
stresses, globally repressed translation initiation leads to stress granule (SG) formation …

Arabidopsis N6-methyladenosine reader CPSF30-L recognizes FUE signals to control polyadenylation site choice in liquid-like nuclear bodies

P Song, J Yang, C Wang, Q Lu, L Shi, S Tayier, G Jia - Molecular plant, 2021 - cell.com
The biological functions of the epitranscriptomic modification N 6-methyladenosine (m 6 A)
in plants are not fully understood. CPSF30-L is a predominant isoform of the polyadenylation …

CPSF30-L-mediated recognition of mRNA m6A modification controls alternative polyadenylation of nitrate signaling-related gene transcripts in Arabidopsis

Y Hou, J Sun, B Wu, Y Gao, H Nie, Z Nie, S Quan… - Molecular plant, 2021 - cell.com
Abstract N 6-methyladenosine (m 6 A), a ubiquitous internal modification of eukaryotic
mRNAs, plays a vital role in almost every aspect of mRNA metabolism. However, there is …

UTR-dependent control of gene expression in plants

AK Srivastava, Y Lu, G Zinta, Z Lang, JK Zhu - Trends in Plant Science, 2018 - cell.com
Throughout their lives, plants sense many developmental and environmental stimuli, and
activation of optimal responses against these stimuli requires extensive transcriptional …

In planta determination of the mRNA-binding proteome of Arabidopsis etiolated seedlings

M Reichel, Y Liao, M Rettel, C Ragan, M Evers… - The Plant …, 2016 - academic.oup.com
RNA binding proteins (RBPs) control the fate and expression of a transcriptome. Despite this
fundamental importance, our understanding of plant RBPs is rudimentary, being mainly …

Comprehensive profiling of rhizome‐associated alternative splicing and alternative polyadenylation in moso bamboo (Phyllostachys edulis)

T Wang, H Wang, D Cai, Y Gao, H Zhang… - The Plant …, 2017 - Wiley Online Library
Moso bamboo (Phyllostachys edulis) represents one of the fastest‐spreading plants in the
world, due in part to its well‐developed rhizome system. However, the post‐transcriptional …

Marking RNA: m6A writers, readers, and functions in Arabidopsis

M Reichel, T Köster, D Staiger - Journal of molecular cell …, 2019 - academic.oup.com
Abstract N6-methyladenosine (m6A) emerges as an important modification in eukaryotic
mRNAs. m6A has first been reported in 1974, and its functional significance in mammalian …

PacBio full‐length cDNA sequencing integrated with RNA‐seq reads drastically improves the discovery of splicing transcripts in rice

G Zhang, M Sun, J Wang, M Lei, C Li, D Zhao… - The Plant …, 2019 - Wiley Online Library
In eukaryotes, alternative splicing (AS) greatly expands the diversity of transcripts. However,
it is challenging to accurately determine full‐length splicing isoforms. Recently, more studies …