Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream production

JA Williams, AE Carnes, CP Hodgson - Biotechnology advances, 2009 - Elsevier
Critical molecular and cellular biological factors impacting design of licensable DNA vaccine
vectors that combine high yield and integrity during bacterial production with increased …

Targeting plasmid‐encoded proteins to the antigen presentation pathways

JA Leifert, MP Rodriguez‐Carreno… - Immunological …, 2004 - Wiley Online Library
The antigen presentation pathways constitute a fulcrum on which adaptive immunity is
balanced, and their manipulation should allow us to induce designer immune responses …

Adjuvant effect of multi-CpG motifs on an HIV-1 DNA vaccine

Y Kojima, KQ Xin, T Ooki, K Hamajima, T Oikawa… - Vaccine, 2002 - Elsevier
Synthetic oligodeoxynucleotides (ODN) containing unmethylated CpG motifs trigger an
immune response characterized by the activation of B cells, NK cells and monocytes …

Multimeric soluble CD40 ligand and GITR ligand as adjuvants for human immunodeficiency virus DNA vaccines

GW Stone, S Barzee, V Snarsky, K Kee… - Journal of …, 2006 - Am Soc Microbiol
For use in humans, human immunodeficiency virus (HIV) DNA vaccines may need to include
immunostimulatory adjuvant molecules. CD40 ligand (CD40L), a member of the tumor …

Hemagglutinin (HA) proteins from H1 and H3 serotypes of influenza A viruses require different antigen designs for the induction of optimal protective antibody …

S Wang, J Taaffe, C Parker, A Solórzano, H Cao… - Journal of …, 2006 - Am Soc Microbiol
Effective antibody responses provide crucial immunity against influenza virus infection. The
hemagglutinin (HA) protein is the major target of protective antibody responses induced by …

HIV-1 p55Gag encoded in the lysosome-associated membrane protein-1 as a DNA plasmid vaccine chimera is highly expressed, traffics to the major histocompatibility …

ETA Marques, P Chikhlikar, LB De Arruda… - Journal of Biological …, 2003 - ASBMB
Several genetic vaccines encoding antigen chimeras containing the lysosome-associated
membrane protein (LAMP) translocon, transmembrane, and cytoplasmic domain sequences …

Mucosal HIV-1 pox virus prime-boost immunization induces high-avidity CD8+ T cells with regime-dependent cytokine/granzyme B profiles

C Ranasinghe, SJ Turner, C McArthur… - The Journal of …, 2007 - journals.aai.org
The quality of virus-specific CD8+ CTL immune responses generated by mucosal and
systemic poxvirus prime-boost vaccines were evaluated in terms of T cell avidity and single …

Recombinant adeno-associated virus vectors induce functionally impaired transgene product–specific CD8+ T cells in mice

SW Lin, SE Hensley, N Tatsis… - The Journal of …, 2007 - Am Soc Clin Investig
Recombinant adeno-associated virus (rAAV) vectors were used in human trials as carriers of
vaccines for HIV-1 after encouraging preclinical results. However, the clinical trials yielded …

Improved expression of secretory and trimeric proteins in mammalian cells via the introduction of a new trimer motif and a mutant of the tPA signal sequence

JY Wang, WT Song, Y Li, WJ Chen, D Yang… - Applied microbiology …, 2011 - Springer
Ideal immunogenicity in antigens is a prerequisite to eliciting a sufficiently strong immune
and memory response via either DNA or protein vaccines. To improve immunogenicity …

Immunization with plasmid DNA encoding influenza A virus nucleoprotein fused to a tissue plasminogen activator signal sequence elicits strong immune responses …

M Luo, P Tao, J Li, S Zhou, D Guo, Z Pan - Journal of virological methods, 2008 - Elsevier
DNA vaccination is an effective means of eliciting both humoral and cellular immunity. Most
of influenza vaccines targeted at hemagglutinin (HA) show efficient immunogenicity for …