The next generation of CRISPR–Cas technologies and applications
A Pickar-Oliver, CA Gersbach - Nature reviews Molecular cell biology, 2019 - nature.com
The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …
The biology of CRISPR-Cas: backward and forward
F Hille, H Richter, SP Wong, M Bratovič, S Ressel… - Cell, 2018 - cell.com
In bacteria and archaea, clustered regularly interspaced short palindromic repeats
(CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system …
(CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system …
Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C: G-to-T: A base editors with higher efficiency and product purity
We recently developed base editing, the programmable conversion of target C: G base pairs
to T: A without inducing double-stranded DNA breaks (DSBs) or requiring homology …
to T: A without inducing double-stranded DNA breaks (DSBs) or requiring homology …
Diversity and evolution of class 2 CRISPR–Cas systems
Class 2 CRISPR–Cas systems are characterized by effector modules that consist of a single
multidomain protein, such as Cas9 or Cpf1. We designed a computational pipeline for the …
multidomain protein, such as Cas9 or Cpf1. We designed a computational pipeline for the …
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Current genome-editing technologies introduce double-stranded (ds) DNA breaks at a target
locus as the first step to gene correction,. Although most genetic diseases arise from point …
locus as the first step to gene correction,. Although most genetic diseases arise from point …
The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA
I Fonfara, H Richter, M Bratovič, A Le Rhun… - Nature, 2016 - nature.com
CRISPR–Cas systems that provide defence against mobile genetic elements in bacteria and
archaea have evolved a variety of mechanisms to target and cleave RNA or DNA. The well …
archaea have evolved a variety of mechanisms to target and cleave RNA or DNA. The well …
[HTML][HTML] Biology and applications of CRISPR systems: harnessing nature's toolbox for genome engineering
Bacteria and archaea possess a range of defense mechanisms to combat plasmids and viral
infections. Unique among these are the CRISPR-Cas (clustered regularly interspaced short …
infections. Unique among these are the CRISPR-Cas (clustered regularly interspaced short …
Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems
BACKGROUND Prokaryotes have evolved multiple systems to combat invaders such as
viruses and plasmids. Examples of such defense systems include receptor masking …
viruses and plasmids. Examples of such defense systems include receptor masking …
CRISPR/cas systems redefine nucleic acid detection: principles and methods
M Wang, R Zhang, J Li - Biosensors and Bioelectronics, 2020 - Elsevier
Methods that enable rapid, sensitive and specific analyses of nucleic acid sequences have
positive effects on precise disease diagnostics and effective clinical treatments by providing …
positive effects on precise disease diagnostics and effective clinical treatments by providing …
A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
Although polymerase chain reaction (PCR) is the most widely used method for DNA
amplification, the requirement of thermocycling limits its non-laboratory applications …
amplification, the requirement of thermocycling limits its non-laboratory applications …