RNA interference technology in crop protection against arthropod pests, pathogens and nematodes

M Zotti, EA Dos Santos, D Cagliari… - Pest management …, 2018 - Wiley Online Library
Scientists have made significant progress in understanding and unraveling several aspects
of double‐stranded RNA (dsRNA)‐mediated gene silencing during the last two decades …

[HTML][HTML] RNA-based technologies for insect control in plant production

S Liu, M Jaouannet, DMA Dempsey, J Imani… - Biotechnology …, 2020 - Elsevier
RNA interference (RNAi) is a biological process in which small RNA (sRNA) molecules
sequence-specifically silence gene expression at the transcriptional or post-transcriptional …

[HTML][HTML] Management of pest insects and plant diseases by non-transformative RNAi

D Cagliari, NP Dias, DM Galdeano… - Frontiers in plant …, 2019 - frontiersin.org
Since the discovery of RNA interference (RNAi), scientists have made significant progress
towards the development of this unique technology for crop protection. The RNAi …

Tobacco rattle virus–based virus-induced gene silencing in Nicotiana benthamiana

M Senthil-Kumar, KS Mysore - Nature protocols, 2014 - nature.com
Tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) is widely used in
various plant species to downregulate the expression of a target plant gene. TRV is a …

[HTML][HTML] Manipulating the biosynthesis of bioactive compound alkaloids for next-generation metabolic engineering in opium poppy using CRISPR-Cas 9 genome …

Y Alagoz, T Gurkok, B Zhang, T Unver - Scientific reports, 2016 - nature.com
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated9
(Cas9) endonuclease system is a powerful RNA-guided genome editing tool. CRISPR/Cas9 …

[HTML][HTML] Validation of Reference Genes for Gene Expression Studies in Virus-Infected Nicotiana benthamiana Using Quantitative Real-Time PCR

D Liu, L Shi, C Han, J Yu, D Li, Y Zhang - 2012 - journals.plos.org
Nicotiana benthamiana is the most widely-used experimental host in plant virology. The
recent release of the draft genome sequence for N. benthamiana consolidates its role as a …

[HTML][HTML] Autophagy regulates programmed cell death during the plant innate immune response

Y Liu, M Schiff, K Czymmek, Z Tallóczy, B Levine… - Cell, 2005 - cell.com
The plant innate immune response includes the hypersensitive response (HR), a form of
programmed cell death (PCD). PCD must be restricted to infection sites to prevent the HR …

Gene silencing in plants using artificial microRNAs and other small RNAs

S Ossowski, R Schwab, D Weigel - The Plant Journal, 2008 - Wiley Online Library
Comprehensive analysis of gene function requires the detailed examination of mutant
alleles. In Arabidopsis thaliana, large collections of sequence‐indexed insertion and …

Plant innate immunity: an updated insight into defense mechanism

M Muthamilarasan, M Prasad - Journal of biosciences, 2013 - Springer
Plants are invaded by an array of pathogens of which only a few succeed in causing
disease. The attack by others is countered by a sophisticated immune system possessed by …

Silencing of hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase affects phenylpropanoid biosynthesis

L Hoffmann, S Besseau, P Geoffroy… - The Plant …, 2004 - academic.oup.com
The hydroxyl group in the 3-position of the phenylpropanoid compounds is introduced at the
level of coumarate shikimate/quinate esters, whose synthesis implicates an acyltransferase …