Metabolic engineering: methodologies and applications
Metabolic engineering aims to improve the production of economically valuable molecules
through the genetic manipulation of microbial metabolism. While the discipline is a little over …
through the genetic manipulation of microbial metabolism. While the discipline is a little over …
[HTML][HTML] Dynamic control in metabolic engineering: Theories, tools, and applications
Metabolic engineering has allowed the production of a diverse number of valuable
chemicals using microbial organisms. Many biological challenges for improving bio …
chemicals using microbial organisms. Many biological challenges for improving bio …
Control theory meets synthetic biology
The past several years have witnessed an increased presence of control theoretic concepts
in synthetic biology. This review presents an organized summary of how these control …
in synthetic biology. This review presents an organized summary of how these control …
Biosystems design by machine learning
Biosystems such as enzymes, pathways, and whole cells have been increasingly explored
for biotechnological applications. However, the intricate connectivity and resulting …
for biotechnological applications. However, the intricate connectivity and resulting …
Formulation, construction and analysis of kinetic models of metabolism: A review of modelling frameworks
PA Saa, LK Nielsen - Biotechnology advances, 2017 - Elsevier
Kinetic models are critical to predict the dynamic behaviour of metabolic networks.
Mechanistic kinetic models for large networks remain uncommon due to the difficulty of fitting …
Mechanistic kinetic models for large networks remain uncommon due to the difficulty of fitting …
Current and future modalities of dynamic control in metabolic engineering
Highlights•Two dynamic control modalities have been developed for metabolic
engineering.•The most common modality uses two phases of fermentation: growth and …
engineering.•The most common modality uses two phases of fermentation: growth and …
ROS networks: designs, aging, Parkinson's disease and precision therapies
How the network around ROS protects against oxidative stress and Parkinson's disease
(PD), and how processes at the minutes timescale cause disease and aging after decades …
(PD), and how processes at the minutes timescale cause disease and aging after decades …
Genome-scale architecture of small molecule regulatory networks and the fundamental trade-off between regulation and enzymatic activity
Metabolic flux is in part regulated by endogenous small molecules that modulate the
catalytic activity of an enzyme, eg, allosteric inhibition. In contrast to transcriptional …
catalytic activity of an enzyme, eg, allosteric inhibition. In contrast to transcriptional …
Switchable biocatalytic reactions controlled by interfacial pH changes produced by orthogonal biocatalytic processes
PK Wells, O Smutok, A Melman… - ACS Applied Materials & …, 2021 - ACS Publications
Enzymes immobilized on a nano-structured surface were used to switch the activity of one
enzyme by a local pH change produced by another enzyme. Immobilized amyloglucosidase …
enzyme by a local pH change produced by another enzyme. Immobilized amyloglucosidase …
Programmable gene regulation for metabolic engineering using decoy transcription factor binding sites
Transcription factor decoy binding sites are short DNA sequences that can titrate a
transcription factor away from its natural binding site, therefore regulating gene expression …
transcription factor away from its natural binding site, therefore regulating gene expression …