The E. coli DNA replication fork
DNA replication in Escherichia coli initiates at oriC, the origin of replication and proceeds
bidirectionally, resulting in two replication forks that travel in opposite directions from the …
bidirectionally, resulting in two replication forks that travel in opposite directions from the …
IHF and Fis as Escherichia coli Cell Cycle Regulators: Activation of the Replication Origin oriC and the Regulatory Cycle of the DnaA Initiator
K Kasho, S Ozaki, T Katayama - International journal of molecular …, 2023 - mdpi.com
This review summarizes current knowledge about the mechanisms of timely binding and
dissociation of two nucleoid proteins, IHF and Fis, which play fundamental roles in the …
dissociation of two nucleoid proteins, IHF and Fis, which play fundamental roles in the …
Single-stranded DNA recruitment mechanism in replication origin unwinding by DnaA initiator protein and HU, an evolutionary ubiquitous nucleoid protein
R Yoshida, S Ozaki, H Kawakami… - Nucleic acids …, 2023 - academic.oup.com
The Escherichia coli replication origin oriC contains the initiator ATP-DnaA-Oligomerization
Region (DOR) and its flanking duplex unwinding element (DUE). In the Left-DOR subregion …
Region (DOR) and its flanking duplex unwinding element (DUE). In the Left-DOR subregion …
DnaB helicase dynamics in bacterial DNA replication resolved by single-molecule studies
RR Spinks, LM Spenkelink, SA Stratmann… - Nucleic acids …, 2021 - academic.oup.com
Abstract In Escherichia coli, the DnaB helicase forms the basis for the assembly of the DNA
replication complex. The stability of DnaB at the replication fork is likely important for …
replication complex. The stability of DnaB at the replication fork is likely important for …
Complete genome sequence of the Clostridium difficile laboratory strain 630Δ erm reveals differences from strain 630, including translocation of the mobile element …
Background Clostridium difficile strain 630Δ erm is a spontaneous erythromycin sensitive
derivative of the reference strain 630 obtained by serial passaging in antibiotic-free media. It …
derivative of the reference strain 630 obtained by serial passaging in antibiotic-free media. It …
Single-molecule live cell imaging of Rep reveals the dynamic interplay between an accessory replicative helicase and the replisome
DNA replication must cope with nucleoprotein barriers that impair efficient replisome
translocation. Biochemical and genetic studies indicate accessory helicases play essential …
translocation. Biochemical and genetic studies indicate accessory helicases play essential …
[HTML][HTML] Bacterial replisomes
ZQ Xu, NE Dixon - Current opinion in structural biology, 2018 - Elsevier
Highlights•Cryo-EM structures of Escherichia coli DNA polymerase III complexes.•Bacterial
polymerases quickly exchange at replication forks.•Bacterial replisomes can work in a …
polymerases quickly exchange at replication forks.•Bacterial replisomes can work in a …
Establishing a biochemical understanding of the initiation of chromosome replication in bacteria
B Stillman - Proceedings of the National Academy of …, 2024 - National Acad Sciences
In the mid-1950s, Arthur Kornberg elucidated the enzymatic synthesis of DNA by DNA
polymerase, for which he was recognized with the 1959 Nobel Prize in Physiology or …
polymerase, for which he was recognized with the 1959 Nobel Prize in Physiology or …
Regulatory dynamics in the ternary DnaA complex for initiation of chromosomal replication in Escherichia coli
Y Sakiyama, K Kasho, Y Noguchi… - Nucleic acids …, 2017 - academic.oup.com
In Escherichia coli, the level of the ATP–DnaA initiator is increased temporarily at the time of
replication initiation. The replication origin, oriC, contains a duplex-unwinding element …
replication initiation. The replication origin, oriC, contains a duplex-unwinding element …
[HTML][HTML] Divergent distributions of inverted repeats and G-quadruplex forming sequences in Saccharomyces cerevisiae
M Čutová, J Manta, O Porubiaková, P Kaura, J Šťastný… - Genomics, 2020 - Elsevier
The importance of DNA structure in the regulation of basic cellular processes is an emerging
field of research. Among local non-B DNA structures, inverted repeat (IR) sequences that …
field of research. Among local non-B DNA structures, inverted repeat (IR) sequences that …