Fluorescence microscopy provides an efficient and unique approach to study fixed and
living cells because of its versatility, specificity, and high sensitivity. Fluorescence …

The endoplasmic reticulum (ER) has a broad localization throughout the cell and forms
direct physical contacts with all other classes of membranous organelles, including the …

The endoplasmic reticulum (ER) has a quality-control system for'proof-reading'newly
synthesized proteins, so that only native conformers reach their final destinations. Non …

Since the advent of the green fluorescent protein, the subcellular localization, mobility,
transport routes and binding interactions of proteins can be studied in living cells. Live cell …

The ability to visualize, track, and quantify molecules and events in living cells with high
spatial and temporal resolution is essential for understanding biological systems. Only …

▪ Abstract The endoplasmic reticulum (ER) and the Golgi comprise the first two steps in
protein secretion. Vesicular carriers mediate a continuous flux of proteins and lipids between …

The endoplasmic reticulum (ER) is a continuous, highly dynamic membrane compartment
that is crucial for numerous basic cellular functions. The ER stretches from the nuclear …

Experiments with fluorescence recovery after photobleaching (FRAP) started 30 years ago
to visualize the lateral mobility and dynamics of fluorescent proteins in living cells. Its …

Eukaryotic cells cope with endoplasmic reticulum (ER) stress by activating the unfolded
protein response (UPR), a coordinated system of transcriptional and translational controls …

The unfolded protein response (UPR) is induced by proteotoxic stress of the endoplasmic
reticulum (ER). Here we report that ATF6, a major mammalian UPR sensor, is also activated …