Drosophila melanogaster S2 cells for expression of heterologous genes: From gene cloning to bioprocess development

AM Moraes, SAC Jorge, RM Astray, CAT Suazo… - Biotechnology …, 2012 - Elsevier
In the present review we discuss strategies that have been used for heterologous gene
expression in Drosophila melanogaster Schneider 2 (S2) cells using plasmid vectors. Since …

Heat shock 70 kDa protein cognate 5 involved in WSSV toleration of Litopenaeus vannamei

K Yuan, FH Yuan, HH He, HT Bi, SP Weng… - Developmental & …, 2017 - Elsevier
The expression levels of 97 unigenes encoding heat shock proteins of Litopenaeus
vannamei was scanned, and ten of them were significantly induced by white spot syndrome …

Adaptive laboratory evolution of stable insect cell lines for improved HIV-Gag VLPs production

B Fernandes, J Vidigal, R Correia, MJT Carrondo… - Journal of …, 2020 - Elsevier
Adaptive laboratory evolution (ALE) has been extensively used to modulate the phenotype
of industrial model organisms (eg Escherichia. coli and Saccharomyces cerevisae) towards …

Influence of growth temperature on the production of antibody Fab fragments in different microbes: a host comparative analysis

M Dragosits, G Frascotti… - Biotechnology …, 2011 - Wiley Online Library
Microorganisms encounter diverse stress conditions in their native habitats but also during
fermentation processes, which have an impact on industrial process performance. These …

Nomenclature and guideline to express the amount of a membrane protein synthesized in animal cells in view of bioprocess optimization and production monitoring

EFP Augusto, AM Moraes, RAM Piccoli, MF Barral… - Biologicals, 2010 - Elsevier
Studies of a bioprocess optimization and monitoring for protein synthesis in animal cells face
a challenge on how to express in quantitative terms the system performance. It is possible to …

Performance comparison of recombinant Baculovirus and rabies virus-like particles production using two culture platforms

LGO Guardalini, PES Cavalcante, J Leme, RG Mello… - Vaccines, 2022 - mdpi.com
This work aimed to assess, following upstream optimization in Schott flasks, the scalability
from this culture platform to a stirred-tank bioreactor in order to yield rabies-recombinant …

report Miscellaneous Citation| Accession Number

MSPUSDRE Legal - apps.dtic.mil
This Memorandum for the Chairman of the Joint Chiefs of Staff is open analysis of the major
outcomes and implications emerging from the United States Army War College s 25th …

Optimized expression of the antimicrobial protein Gloverin from Galleria mellonella using stably transformed Drosophila melanogaster S2 cells

J Zitzmann, T Weidner, P Czermak - Cytotechnology, 2017 - Springer
Antimicrobial proteins and peptides (AMPs) are valuable as leads in the pharmaceutical
industry for the development of novel anti-infective drugs. Here we describe the efficient …

Production and N-glycosylation of recombinant human cell adhesion molecule L1 from insect cells using the stable expression system. Effect of dimethyl sulfoxide

R Gouveia, S Kandzia, HS Conradt, J Costa - Journal of biotechnology, 2010 - Elsevier
L1 is a cell adhesion molecule that is heavily glycosylated and is essential for normal
development of the central nervous system. In this work, we compare the N-glycosylation of …

[HTML][HTML] Rabies virus glycoprotein expression in Drosophila S2 cells. I: design of expression/selection vectors, subpopulations selection and influence of sodium …

MAN Lemos, AS Dos Santos, RM Astray… - Journal of …, 2009 - Elsevier
The cDNA encoding the rabies virus glycoprotein (RVGP) gene was cloned in expression
plasmids under the control of the inductive metallothionein promoter. They were designed in …