Given the highly dynamic nature of mRNA transcription and the potential variables
introduced in sample handling and in the downstream processing steps (Garson et …

Lack of an objective, accurate, and noninvasive embryo assessment strategy remains one of
the major challenges encountered in in vitro fertilization. Cumulus and mural granulosa cells …

Digital PCR (dPCR) has developed considerably since the publication of the Minimum
Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with …

Polymerase chain reaction (PCR) is a basic molecular biology technique with a multiplicity
of uses, including deoxyribonucleic acid cloning and sequencing, functional analysis of …

MicroRNAs are important negative regulators of protein-coding gene expression and have
been studied intensively over the past years. Several measurement platforms have been …

Background: Currently, a lack of consensus exists on how best to perform and interpret
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …

Reverse transcription quantitative PCR (RT-qPCR) is considered today as the gold standard
for accurate, sensitive and fast measurement of gene expression. Unfortunately, what many …

Gene expression analysis of microRNA molecules is becoming increasingly important. In
this study we assess the use of the mean expression value of all expressed microRNAs in a …

Background The analyses of amplification and melting curves have been shown to provide
valuable information on the quality of the individual reactions in quantitative PCR (qPCR) …

Drosophila melanogaster is one of the most important genetic models and techniques such
as reverse transcription quantitative real-time PCR (RT-qPCR) are being employed …