Agnostic metagenomic next-generation sequencing (mNGS) has emerged as a promising
single, universal pathogen detection method for infectious disease diagnostics. This …

Metagenomic next-generation sequencing (mNGS) is increasingly being applied in clinical
laboratories for unbiased culture-independent diagnosis. Whether it can be a next routine …

A fatal case of Japanese encephalitis (JE) occurred in northern Australia in early 2021.
Sequence studies showed that the virus belonged to genotype IV (GIV), a genotype …

Background Current estimates suggest that even in the most resourced settings, the
aetiology of encephalitis is identified in less than half of clinical cases. It is acknowledged …

Metagenomic shotgun sequencing has the potential to transform how serious infections are
diagnosed by offering universal, culture-free pathogen detection. This may be especially …

Purpose The application of metagenomic next-generation sequencing (mNGS) in the
diagnosis of tuberculous meningitis (TBM) remains poorly characterized. Here, we …

Objective: To assess the clinical utilization and performance of the FilmArray® Meningitis/
Encephalitis (ME) multiplex polymerase chain reaction (PCR) panel in a hospital setting …

The purpose of this study was to develop and optimize different processing, extraction,
amplification, and sequencing methods for metagenomic next-generation sequencing …

Background Brucellosis is the most common zoonotic infection in the world. Brucellosis with
nervous system involvement is known as 'neurobrucellosis'(NB). The diagnosis of NB is …

Metagenomic sequencing is a powerful technique that enables detection of the full spectrum
of pathogens present in any specimen in a single test. Hence, metagenomics is increasingly …