Mutational fitness landscape and drug resistance
Robust technology has been developed to systematically quantify fitness landscapes that
provide valuable opportunities to improve our understanding of drug resistance and define …
provide valuable opportunities to improve our understanding of drug resistance and define …
CRISPR/Cas9 recombineering‐mediated deep mutational scanning of essential genes in Escherichia coli
A Choudhury, JA Fenster, RG Fankhauser… - Molecular Systems …, 2020 - embopress.org
Deep mutational scanning can provide significant insights into the function of essential
genes in bacteria. Here, we developed a high‐throughput method for mutating essential …
genes in bacteria. Here, we developed a high‐throughput method for mutating essential …
Small molecule regulated sgRNAs enable control of genome editing in E. coli by Cas9
CRISPR-Cas9 has led to great advances in gene editing for a broad spectrum of
applications. To further the utility of Cas9 there have been efforts to achieve temporal control …
applications. To further the utility of Cas9 there have been efforts to achieve temporal control …
Predicting drug resistance using deep mutational scanning
Drug resistance is a major healthcare challenge, resulting in a continuous need to develop
new inhibitors. The development of these inhibitors requires an understanding of the …
new inhibitors. The development of these inhibitors requires an understanding of the …
Potential repurposing of four FDA approved compounds with antiplasmodial activity identified through proteome scale computational drug discovery and in vitro assay
BN Diallo, T Swart, HC Hoppe, Ö Tastan Bishop… - Scientific Reports, 2021 - nature.com
Malaria elimination can benefit from time and cost-efficient approaches for antimalarials
such as drug repurposing. In this work, 796 DrugBank compounds were screened against …
such as drug repurposing. In this work, 796 DrugBank compounds were screened against …
Inhibition of DXR in the MEP pathway with lipophilic N-alkoxyaryl FR900098 analogs
D Bague, R Wang, D Hodge, MO Mikati… - RSC Medicinal …, 2024 - pubs.rsc.org
In Mycobacterium tuberculosis (Mtb) and Plasmodium falciparum (Pf), the methylerythritol
phosphate (MEP) pathway is responsible for isoprene synthesis. This pathway and its …
phosphate (MEP) pathway is responsible for isoprene synthesis. This pathway and its …
Antimicrobial prodrug activation by the staphylococcal glyoxalase GloB
With the rising prevalence of multidrug resistance, there is an urgent need to develop novel
antibiotics. Many putative antibiotics demonstrate promising in vitro potency but fail in vivo …
antibiotics. Many putative antibiotics demonstrate promising in vitro potency but fail in vivo …
Antibacterial Activity of Peptide Derivatives of Phosphinothricin against Multidrug-Resistant Klebsiella pneumoniae
MV Demiankova, F Giovannercole, MA Khomutov… - Molecules, 2023 - mdpi.com
The fast spread of bacteria that are resistant to many classes of antibiotics (multidrug
resistant) is a global threat to human and animal health with a worrisome scenario ahead …
resistant) is a global threat to human and animal health with a worrisome scenario ahead …
[HTML][HTML] Enzymatic Synthesis of Biologically Active H-Phosphinic Analogue of α-Ketoglutarate
VL Filonov, MA Khomutov, YV Tkachev, AV Udod… - Biomolecules, 2024 - mdpi.com
Amino acid analogues with a phosphorus-containing moiety replacing the carboxylic group
are promising sources of biologically active compounds. The H-phosphinic group, with …
are promising sources of biologically active compounds. The H-phosphinic group, with …
Highly efficient libraries design for saturation mutagenesis
Saturation mutagenesis is a semi-rational approach for protein engineering where sites are
saturated either entirely or partially to include amino acids of interest. We previously …
saturated either entirely or partially to include amino acids of interest. We previously …