Modeling the homologous recombination process: Methods, successes and challenges
Homologous recombination (HR) is a fundamental process common to all species. HR aims
to faithfully repair DNA double strand breaks. HR involves the formation of nucleoprotein …
to faithfully repair DNA double strand breaks. HR involves the formation of nucleoprotein …
Engineering polymerases for new functions
TA Coulther, HR Stern, PJ Beuning - Trends in biotechnology, 2019 - cell.com
DNA polymerases are critical tools in biotechnology, enabling efficient and accurate
amplification of DNA templates, yet many desired functions are not readily available in …
amplification of DNA templates, yet many desired functions are not readily available in …
Fast native-SAD phasing for routine macromolecular structure determination
We describe a data collection method that uses a single crystal to solve X-ray structures by
native SAD (single-wavelength anomalous diffraction). We solved the structures of 11 real …
native SAD (single-wavelength anomalous diffraction). We solved the structures of 11 real …
Structure and dynamics of the CRISPR–Cas9 catalytic complex
G Palermo - Journal of Chemical Information and Modeling, 2019 - ACS Publications
CRISPR–Cas9 is a bacterial immune system with exciting applications for genome editing.
In spite of extensive experimental characterization, the active site chemistry of the RuvC …
In spite of extensive experimental characterization, the active site chemistry of the RuvC …
cryo-EM structures of the E. coli replicative DNA polymerase reveal its dynamic interactions with the DNA sliding clamp, exonuclease and τ
R Fernandez-Leiro, J Conrad, SHW Scheres… - Elife, 2015 - elifesciences.org
The replicative DNA polymerase PolIIIα from Escherichia coli is a uniquely fast and
processive enzyme. For its activity it relies on the DNA sliding clamp β, the proofreading …
processive enzyme. For its activity it relies on the DNA sliding clamp β, the proofreading …
Pyrophosphate hydrolysis is an intrinsic and critical step of the DNA synthesis reaction
DNA synthesis by DNA polymerases (dPols) is central to duplication and maintenance of the
genome in all living organisms. dPols catalyze the formation of a phosphodiester bond …
genome in all living organisms. dPols catalyze the formation of a phosphodiester bond …
Novel autoproteolytic and DNA-damage sensing components in the bacterial SOS response and oxidized methylcytosine-induced eukaryotic DNA demethylation …
Abstract The bacterial SOS response is an elaborate program for DNA repair, cell cycle
regulation and adaptive mutagenesis under stress conditions. Using sensitive sequence …
regulation and adaptive mutagenesis under stress conditions. Using sensitive sequence …
Structure and function of extreme TLS DNA polymerase TTEDbh from Thermoanaerobacter tengcongensis
LF Tian, H Gao, S Yang, YP Liu, M Li, W Xu… - International Journal of …, 2023 - Elsevier
Translesion synthesis (TLS) is a kind of DNA repair that maintains the stability of the genome
and ensures the normal growth of life in cells under emergencies. Y-family DNA …
and ensures the normal growth of life in cells under emergencies. Y-family DNA …
Probing conformational transitions towards mutagenic Watson–Crick-like G·T mismatches using off-resonance sugar carbon R1ρ relaxation dispersion
NMR off-resonance R 1ρ relaxation dispersion measurements on base carbon and nitrogen
nuclei have revealed that wobble G· T/U mismatches in DNA and RNA duplexes exist in …
nuclei have revealed that wobble G· T/U mismatches in DNA and RNA duplexes exist in …
Reactive oxygen species play an important role in the bactericidal activity of quinolone antibiotics
Recent studies posit that reactive oxygen species (ROS) contribute to the cell lethality of
bactericidal antibiotics. However, this conjecture has been challenged and remains …
bactericidal antibiotics. However, this conjecture has been challenged and remains …