Single-cell transcriptomics for the assessment of cardiac disease

AMA Miranda, V Janbandhu, H Maatz… - Nature Reviews …, 2023 - nature.com
Cardiovascular disease is the leading cause of death globally. An advanced understanding
of cardiovascular disease mechanisms is required to improve therapeutic strategies and …

Maturing differentiated human pluripotent stem cells in vitro: methods and challenges

D Ottaviani, M Ter Huurne, DA Elliott, M Bellin… - …, 2023 - journals.biologists.com
Human pluripotent stem cells (hPSCs), derived from individuals or genetically modified with
disease-related mutations and variants, have revolutionised studies of human disease …

Integrated multi-omic characterization of congenital heart disease

MC Hill, ZA Kadow, H Long, Y Morikawa, TJ Martin… - Nature, 2022 - nature.com
The heart, the first organ to develop in the embryo, undergoes complex morphogenesis that
when defective results in congenital heart disease (CHD). With current therapies, more than …

Integration mapping of cardiac fibroblast single-cell transcriptomes elucidates cellular principles of fibrosis in diverse pathologies

R Patrick, V Janbandhu, V Tallapragada, SSM Tan… - Science …, 2024 - science.org
Single-cell technology has allowed researchers to probe tissue complexity and dynamics at
unprecedented depth in health and disease. However, the generation of high-dimensionality …

[HTML][HTML] Vascular cells improve functionality of human cardiac organoids

HK Voges, SR Foster, L Reynolds, BL Parker… - Cell Reports, 2023 - cell.com
Crosstalk between cardiac cells is critical for heart performance. Here we show that vascular
cells within human cardiac organoids (hCOs) enhance their maturation, force of contraction …

propeller: testing for differences in cell type proportions in single cell data

B Phipson, CB Sim, ER Porrello, AW Hewitt… - …, 2022 - academic.oup.com
Abstract Motivation Single cell RNA-Sequencing (scRNA-seq) has rapidly gained popularity
over the last few years for profiling the transcriptomes of thousands to millions of single cells …

Physiological calcium combined with electrical pacing accelerates maturation of human engineered heart tissue

S Shen, LR Sewanan, S Shao, SS Halder, P Stankey… - Stem cell reports, 2022 - cell.com
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have wide
potential application in basic research, drug discovery, and regenerative medicine, but …

Electrically Conductive Collagen‐PEDOT: PSS Hydrogel Prevents Post‐Infarct Cardiac Arrhythmia and Supports hiPSC‐Cardiomyocyte Function

K Roshanbinfar, M Schiffer, E Carls… - Advanced …, 2024 - Wiley Online Library
Myocardial infarction (MI) causes cell death, disrupts electrical activity, triggers arrhythmia,
and results in heart failure, whereby 50–60% of MI‐associated deaths manifest as sudden …

Engineering the maturation of stem cell-derived cardiomyocytes

Y Hong, Y Zhao, H Li, Y Yang, M Chen… - … in Bioengineering and …, 2023 - frontiersin.org
The maturation of human stem cell-derived cardiomyocytes (hSC-CMs) has been a major
challenge to further expand the scope of their application. Over the past years, several …

Sex-dependent transcription of cardiac electrophysiology and links to acetylation modifiers based on the GTEx database

MP Pressler, A Horvath, E Entcheva - Frontiers in Cardiovascular …, 2022 - frontiersin.org
Development of safer drugs based on epigenetic modifiers, eg, histone deacetylase
inhibitors (HDACi), requires better understanding of their effects on cardiac …