Real-time PCR for mRNA quantitation
ML Wong, JF Medrano - Biotechniques, 2005 - Taylor & Francis
Real-time PCR has become one of the most widely used methods of gene quantitation
because it has a large dynamic range, boasts tremendous sensitivity, can be highly …
because it has a large dynamic range, boasts tremendous sensitivity, can be highly …
[HTML][HTML] Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction
SA Bustin, T Nolan - Journal of biomolecular techniques: JBT, 2004 - ncbi.nlm.nih.gov
Polymerase chain reaction (PCR)-based assays can target either DNA (the genome) or RNA
(the transcriptome). Targeting the genome generates robust data that are informative and …
(the transcriptome). Targeting the genome generates robust data that are informative and …
Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR
N Silver, S Best, J Jiang, SL Thein - BMC molecular biology, 2006 - Springer
Background Control genes, which are often referred to as housekeeping genes, are
frequently used to normalise mRNA levels between different samples. However, the …
frequently used to normalise mRNA levels between different samples. However, the …
Guideline to reference gene selection for quantitative real-time PCR
A Radonić, S Thulke, IM Mackay, O Landt… - Biochemical and …, 2004 - Elsevier
Today, quantitative real-time PCR is the method of choice for rapid and reliable
quantification of mRNA transcription. However, for an exact comparison of mRNA …
quantification of mRNA transcription. However, for an exact comparison of mRNA …
Validation of housekeeping genes for normalizing RNA expression in real-time PCR
Analysis of RNA expression using techniques like real-time PCR has traditionally used
reference or housekeeping genes to control for error between samples. This practice is …
reference or housekeeping genes to control for error between samples. This practice is …
Evaluation of candidate control genes for diagnosis and residual disease detection in leukemic patients using 'real-time'quantitative reverse-transcriptase polymerase …
E Beillard, N Pallisgaard, VHJ Van der Velden, W Bi… - Leukemia, 2003 - nature.com
Real-time quantitative RT-PCR (RQ-PCR) is a sensitive tool to monitor minimal residual
disease (MRD) in leukemic patients through the amplification of a fusion gene (FG) …
disease (MRD) in leukemic patients through the amplification of a fusion gene (FG) …
Human housekeeping genes are compact
E Eisenberg, EY Levanon - TRENDS in Genetics, 2003 - cell.com
We identify a set of 575 human genes that are expressed in all conditions tested in a publicly
available database of microarray results. Based on this common occurrence, the set is …
available database of microarray results. Based on this common occurrence, the set is …
Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon
Background Salmonid fishes are among the most widely studied model fish species but
reports on systematic evaluation of reference genes in qRT-PCR studies is lacking. Results …
reports on systematic evaluation of reference genes in qRT-PCR studies is lacking. Results …
Evaluation of potential reference genes for reverse transcription-qPCR studies of physiological responses in Drosophila melanogaster
Drosophila melanogaster is one of the most important genetic models and techniques such
as reverse transcription quantitative real-time PCR (RT-qPCR) are being employed …
as reverse transcription quantitative real-time PCR (RT-qPCR) are being employed …
Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue …
C Tricarico, P Pinzani, S Bianchi, M Paglierani… - Analytical …, 2002 - Elsevier
Careful normalization is essential when using quantitative reverse transcription polymerase
chain reaction assays to compare mRNA levels between biopsies from different individuals …
chain reaction assays to compare mRNA levels between biopsies from different individuals …