An improved method for gene replacement in Pseudomonas aeruginosa was developed.
The method employs several new gene replacement vectors that incorporate (1) the …

Pseudomonas aeruginosa causes chronic biofilm infections, and its ability to attach to
surfaces and other cells is important for biofilm formation and maintenance. Mutations in a …

Bacteria communicate with each other to coordinate expression of specific genes in a cell
density-dependent fashion, a phenomenon called quorum sensing and response. Although …

The bacterial wilt pathogen Ralstonia solanacearum produces three extracellular
polygalacturonases (PGs): PehA, PehB, and PehC. All three PGs hydrolyze pectin's …

Bacteria inhabiting biofilms usually produce one or more polysaccharides that provide a
hydrated scaffolding to stabilize and reinforce the structure of the biofilm, mediate cell-cell …

The nucleotide sequence of the 1.9-kb PstI fragment from pRO1614, that allows stable
maintenance ofpMB1 (ColE1)-based cloning vectors in Pseudomonas, was determined …

The opportunistic pathogenic bacterium Pseudomonas aeruginosa uses quorum-sensing
signaling systems as global regulators of virulence genes. There are two quorum-sensing …

The assembly of iron-sulfur (Fe-S) clusters is mediated by complex machinery. In several
proteobacteria, this process involves ISC (Fe-S cluster assembly) machinery composed of at …

ABSTRACT A series of modular mini-transposon derivatives which permit the rapid cloning
and mapping of the DNA flanking the minitransposon's site of insertion has been developed …

Caulobacter crescentus is widely used as a powerful model system for the study of
prokaryotic cell biology and development. Analysis of this organism is complicated by a …