Directed evolution: methodologies and applications

Y Wang, P Xue, M Cao, T Yu, ST Lane… - Chemical reviews, 2021 - ACS Publications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

Application of combinatorial optimization strategies in synthetic biology

G Naseri, MAG Koffas - Nature communications, 2020 - nature.com
In the first wave of synthetic biology, genetic elements, combined into simple circuits, are
used to control individual cellular functions. In the second wave of synthetic biology, the …

A biological camera that captures and stores images directly into DNA

CK Lim, JW Yeoh, AA Kunartama, WS Yew… - Nature …, 2023 - nature.com
The increasing integration between biological and digital interfaces has led to heightened
interest in utilizing biological materials to store digital data, with the most promising one …

A red light–responsive photoswitch for deep tissue optogenetics

Y Kuwasaki, K Suzuki, G Yu, S Yamamoto… - Nature …, 2022 - nature.com
Red light penetrates deep into mammalian tissues and has low phototoxicity, but few
optogenetic tools that use red light have been developed. Here we present MagRed, a red …

Multidimensional characterization of inducible promoters and a highly light-sensitive LOV-transcription factor

V Gligorovski, A Sadeghi, SJ Rahi - Nature Communications, 2023 - nature.com
The ability to independently control the expression of different genes is important for
quantitative biology. Using budding yeast, we characterize GAL1pr, GALL, MET3pr, CUP1pr …

Sensing the future of bio-informational engineering

TA Dixon, TC Williams, IS Pretorius - Nature Communications, 2021 - nature.com
The practices of synthetic biology are being integrated into 'multiscale'designs enabling two-
way communication across organic and inorganic information substrates in biological, digital …

Combinatorial optimization of gene expression through recombinase-mediated promoter and terminator shuffling in yeast

C Cautereels, J Smets, P Bircham… - Nature …, 2024 - nature.com
Microbes are increasingly employed as cell factories to produce biomolecules. This often
involves the expression of complex heterologous biosynthesis pathways in host strains …

The red edge: bilin-binding photoreceptors as optogenetic tools and fluorescence reporters

K Tang, HM Beyer, MD Zurbriggen… - Chemical …, 2021 - ACS Publications
This review adds the bilin-binding phytochromes to the Chemical Reviews thematic issue
“Optogenetics and Photopharmacology”. The work is structured into two parts. We first …

[PDF][PDF] Tasting the terroir of wine yeast innovation

IS Pretorius - FEMS yeast research, 2020 - academic.oup.com
Wine is an archetypal traditional fermented beverage with strong territorial and socio-cultural
connotations. Its 7000 year history is patterned by a tradition of innovation. Every value …

Heterozygous diploid and interspecies SCRaMbLEing

MJ Shen, Y Wu, K Yang, Y Li, H Xu, H Zhang… - Nature …, 2018 - nature.com
Abstract SCRaMbLE (Synthetic Chromosome Rearrangement and Modification by LoxP-
mediated Evolution) is a genome restructuring technique that can be used in synthetic …