Fluorescent DNA-based enzyme sensors

N Dai, ET Kool - Chemical Society Reviews, 2011 - pubs.rsc.org
Fluorescent sensors that make use of DNA structures have become widely useful in
monitoring enzymatic activities. Early studies focused primarily on enzymes that naturally …

Signal amplification by enzymatic tools for nucleic acids

P Miao, Y Tang, B Wang, J Yin, L Ning - TrAC Trends in Analytical …, 2015 - Elsevier
This review provides a comprehensive overview of current research involving the use of
enzymatic tools for nucleic acids in the fabrication of signal amplification for biosensors. The …

Plasmonic AuNP/gC3N4 Nanohybrid-based Photoelectrochemical Sensing Platform for Ultrasensitive Monitoring of Polynucleotide Kinase Activity Accompanying …

J Zhuang, W Lai, M Xu, Q Zhou… - ACS Applied Materials & …, 2015 - ACS Publications
A convenient and feasible photoelectrochemical (PEC) sensing platform based on gold
nanoparticles-decorated g-C3N4 nanosheets (AuNP/g-C3N4) was designed for highly …

Conjugating a groove-binding motif to an Ir (III) complex for the enhancement of G-quadruplex probe behavior

M Wang, Z Mao, TS Kang, CY Wong, JL Mergny… - Chemical …, 2016 - pubs.rsc.org
In this study, the reported G-quadruplex groove binder benzo [d, e] isoquinoline was linked
to a cyclometallated Ir (III) complex to generate a highly selective DNA probe 1 that retains …

Sensitive and rapid screening of T4 polynucleotide kinase activity and inhibition based on coupled exonuclease reaction and graphene oxide platform

L Lin, Y Liu, X Zhao, J Li - Analytical chemistry, 2011 - ACS Publications
Phosphorylation of DNA with 5′-hydroxyl termini plays a critical role in a majority of normal
cellular events, including DNA recombination, DNA replication, and repair of DNA during …

Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

HZ He, KH Leung, W Wang, DSH Chan… - Chemical …, 2014 - pubs.rsc.org
A label-free, oligonucleotide-based, switch-on luminescence detection method for T4
polynucleotide kinase activity has been developed using a novel G-quadruplex-selective …

Single-molecule detection of polynucleotide kinase based on phosphorylation-directed recovery of fluorescence quenched by au nanoparticles

L Wang, Q Zhang, B Tang, C Zhang - Analytical Chemistry, 2017 - ACS Publications
5′-Polynucleotide kinase such as T4 polynucleotide kinase (T4 PNK) may catalyze the
phosphorylation of 5′-hydroxyl termini in nucleic acids, playing a crucial role in DNA …

Amplified detection of T4 polynucleotide kinase activity by the coupled λ exonuclease cleavage reaction and catalytic assembly of bimolecular beacons

T Hou, X Wang, X Liu, T Lu, S Liu, F Li - Analytical chemistry, 2014 - ACS Publications
The phosphorylation of nucleic acid catalyzed by polynucleotide kinase is an indispensible
procedure involved in many vital cellular activities such as DNA recombination and DNA …

Ultra-specific fluorescence detection of DNA modifying enzymes by dissipation system

J Liu, Y Liu, L Zhang, S Fu, X Su - Biosensors and Bioelectronics, 2022 - Elsevier
Abnormal expression of DNA modifying enzymes (DMEs) is linked to a variety of diseases
including cancers. It is desirable to develop accurate methods for DME detection. However …

A cobalt oxyhydroxide nanoflake-based nanoprobe for the sensitive fluorescence detection of T4 polynucleotide kinase activity and inhibition

Y Cen, Y Yang, RQ Yu, TT Chen, X Chu - Nanoscale, 2016 - pubs.rsc.org
Phosphorylation of nucleic acids with 5′-OH termini catalyzed by polynucleotide kinase
(PNK) is an inevitable process and has been implicated in many important cellular events …