Computer-aided understanding and engineering of enzymatic selectivity
L Wu, L Qin, Y Nie, Y Xu, YL Zhao - Biotechnology Advances, 2022 - Elsevier
Abstract Enzymes offering chemo-, regio-, and stereoselectivity enable the asymmetric
synthesis of high-value chiral molecules. Unfortunately, the drawback that naturally …
synthesis of high-value chiral molecules. Unfortunately, the drawback that naturally …
Recent advances and promises in nitrile hydratase: from mechanism to industrial applications
Nitrile hydratase (NHase, EC 4.2. 1.84) is one type of metalloenzyme participating in the
biotransformation of nitriles into amides. Given its catalytic specificity in amide production …
biotransformation of nitriles into amides. Given its catalytic specificity in amide production …
HotSpot Wizard 3.0: web server for automated design of mutations and smart libraries based on sequence input information
L Sumbalova, J Stourac, T Martinek… - Nucleic Acids …, 2018 - academic.oup.com
HotSpot Wizard is a web server used for the automated identification of hotspots in semi-
rational protein design to give improved protein stability, catalytic activity, substrate …
rational protein design to give improved protein stability, catalytic activity, substrate …
Engineering Saccharomyces cerevisiae for high yield production of α-amyrin via synergistic remodeling of α-amyrin synthase and expanding the storage pool
Y Yu, A Rasool, H Liu, B Lv, P Chang, H Song… - Metabolic …, 2020 - Elsevier
Abstract α-Amyrin is a plant-originated high-valued triterpene that is highly effective against
several pathological ailments. α-Amyrin production by engineered Saccharomyces …
several pathological ailments. α-Amyrin production by engineered Saccharomyces …
Insight into the broadened substrate scope of nitrile hydratase by static and dynamic structure analysis
The narrow substrate scope limits the wide industrial application of enzymes. Here, we
successfully broadened the substrate scope of a nitrile hydratase (NHase) through mutation …
successfully broadened the substrate scope of a nitrile hydratase (NHase) through mutation …
“Toolbox” construction of an extremophilic nitrile hydratase from Streptomyces thermoautotrophicus for the promising industrial production of various amides
J Guo, J Berdychowska, Q Lai, Y Meng, Z Cheng… - International Journal of …, 2022 - Elsevier
Abstract Nitrile hydratase (NHase; EC 4.2. 1.84) is widely used to synthesize the
corresponding amides from nitriles, which is the most successful green biocatalyst …
corresponding amides from nitriles, which is the most successful green biocatalyst …
[HTML][HTML] Structure-oriented engineering of nitrile hydratase: Reshaping of substrate access tunnel and binding pocket for efficient synthesis of cinnamamide
D Ma, Z Cheng, L Han, J Guo, L Peplowski… - International Journal of …, 2024 - Elsevier
Cinnamamide and its derivatives are the most common and important building blocks widely
present in natural products. Currently, nitrile hydratase (NHase, EC 4.2. 1.84) has been …
present in natural products. Currently, nitrile hydratase (NHase, EC 4.2. 1.84) has been …
Programing a cyanide-free transformation of aldehydes to nitriles and one-pot synthesis of amides through tandem chemo-enzymatic cascades
H Zheng, Q Xiao, F Mao, A Wang, M Li, Q Wang… - RSC …, 2022 - pubs.rsc.org
Nitriles are broadly applied to synthesize pharmaceuticals, agrochemicals, and materials
because of their versatile transformation. Although various methods have been developed …
because of their versatile transformation. Although various methods have been developed …
Computational Design of Nitrile Hydratase from Pseudonocardia thermophila JCM3095 for Improved Thermostability
High thermostability and catalytic activity are key properties for nitrile hydratase (NHase, EC
4.2. 1.84) as a well-industrialized catalyst. In this study, rational design was applied to tailor …
4.2. 1.84) as a well-industrialized catalyst. In this study, rational design was applied to tailor …
Substrate access tunnel engineering for improving the catalytic activity of a thermophilic nitrile hydratase toward pyridine and pyrazine nitriles
Z Cheng, S Jiang, Z Zhou - Biochemical and Biophysical Research …, 2021 - Elsevier
Nitrile hydratase (NHase) is able to bio-transform nitriles into amides. As nitrile hydration
being an exothermic reaction, a NHase with high activity and stability is needed for amide …
being an exothermic reaction, a NHase with high activity and stability is needed for amide …